Target-selective joint polymerase chain reaction: A robust and rapid method for high-throughput production of recombinant monoclonal antibodies from single cells

[1]  M. Isobe,et al.  Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells , 2011, BMC biotechnology.

[2]  Tsutomu Obata,et al.  Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array , 2011, Nature Protocols.

[3]  Cécile Crosnier,et al.  A rapid and scalable method for selecting recombinant mouse monoclonal antibodies , 2010, BMC Biology.

[4]  Louis M. Weiner,et al.  Monoclonal antibodies: versatile platforms for cancer immunotherapy , 2010, Nature Reviews Immunology.

[5]  Andrew C. Chan,et al.  Therapeutic antibodies for autoimmunity and inflammation , 2010, Nature Reviews Immunology.

[6]  T. Munro,et al.  A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies. , 2010, Journal of immunological methods.

[7]  M. Isobe,et al.  Minimum Length of Homology Arms Required for Effective Red/ET Recombination , 2009, Bioscience, biotechnology, and biochemistry.

[8]  T. Tiller,et al.  Cloning and expression of murine Ig genes from single B cells. , 2009, Journal of immunological methods.

[9]  T. Kepler,et al.  High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies. , 2009, Journal of virological methods.

[10]  J. D. Capra,et al.  Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen , 2009, Nature Protocols.

[11]  Patrick C. Wilson,et al.  Rapid cloning of high-affinity human monoclonal antibodies against influenza virus , 2008, Nature.

[12]  Marie-Paule Lefranc,et al.  IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis , 2008, Nucleic Acids Res..

[13]  M. Altmeyer,et al.  Quantitative analysis of the binding affinity of poly(ADP-ribose) to specific binding proteins as a function of chain length , 2007, Nucleic acids research.

[14]  R. Buller,et al.  Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase , 2006, Nucleic acids research.

[15]  P. S. Andersen,et al.  Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing. , 2006, Journal of molecular biology.

[16]  P. Telleman,et al.  Amplification of IgG VH and VL (Fab) from single human plasma cells and B cells. , 2000, Nucleic acids research.

[17]  Youming Zhang,et al.  RecE/RecT and Redα/Redβ initiate double-stranded break repair by specifically interacting with their respective partners , 2000, Genes & Development.

[18]  Stephen J. Elledge,et al.  The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes , 1998, Current Biology.

[19]  J. Schrader,et al.  A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[20]  H. Nakano,et al.  In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis. , 2010, Journal of bioscience and bioengineering.

[21]  Michel C Nussenzweig,et al.  Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning. , 2008, Journal of immunological methods.

[22]  A. Stewart,et al.  RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners. , 2000, Genes & development.