Use of zygotic embryo culture to collect germplasm of coconut (Cocos nucifera L.)

The in vitro culture of zygotic embryos solves the problems encountered in transporting and storing coconut seeds (Cocos nucifera L.). especially when obtained under field collecting conditions. Embryo sampling and short-term storage methods have been developed. Direct inoculation into culture on media, enabling embryo growth under axenic conditions. has been successfully carried out under field collecting conditions. An alternative method holds embryos in endosperm cores in a salt solution for brief periods before excision and inoculation into culture in a laboratory. The techniques and equipment used are described in this paper, as well as the subsequent development of the ernbryos and the transfer of plants to natural growing conditions.