Extraction, Characterization and Application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil

Red pigmented bacterial strain isolated from the so il sample of Kharaghoda area, Gujarat was used for the present study. This halophile, Gram negative ba cteria was identified as a Serratia marcescens stra in by 16s rRNA gene sequencing. This bacterium showed salt tolerance up to 12% Nacl. The gene sequence was submitted to NCBI and it is released by named S erratia marcescens KH1R KM035849. The highest extraction of this pigment was yielded in 2:1= 85% Methanol: Acetone mixture among different concentration of different solvents. The Rf value o f the extracted pigment was 0.64-0.96 in TLC method . The maximum absorption spectrum was observed at 535 nm by UV-Visible Spectroscopy. This pigment exhibited antibacterial activity against B.cerus, S .aureus and E.coli with inhibition zone 12 mm, 07 m m and 06 mm respectively. This pigment was showing an tioxidant activity confirmed by DPPH reduction method. The Serratia marcescens KH1R KM035849 strai n is also siderophore producer.

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