Biotransformation of vegetable and fruit processing wastes into yeast biomass enriched with selenium.

Water extracts of cabbage, watermelon, a mixture of residual biomass of green salads and tropical fruits were used for yeast cultivation. These extracts contained from 1420 to 8900 mg/l of dissolved organic matter, and from 600 to 1800 mg/l of nitrogen. pH of the extracts was in the range from 4.1 to 6.4. Biomass concentration of yeast, Saccharomyces cerevisiae CEE 12 grown at 30 degrees C for 96 h in the sterilized extracts without any nutrient supplements was from 6.4 to 8.2 g/l; content of protein was from 40% to 45% of dry biomass. The yield was comparable with the yield of yeast biomass grown in potato dextrose broth. The biomass can be considered as the protein source. Its feed value was enhanced by incorporation of selenium in biomass to the level of 150 microg/g of dry biomass. Therefore, it was recommended to transform the extracts from vegetable and fruit processing wastes into the yeast biomass enriched with selenium.

[1]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[2]  I. Pais,et al.  Preparation of selenium yeasts I. Preparation of selenium-enriched Saccharomyces cerevisiae. , 2000, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[3]  Jeong-Woo Choi,et al.  Control of ethanol concentration in a fed-batch cultivation of Acinetobacter calcoaceticus RAG-1 using a feedback-assisted iterative learning algorithm , 1996 .

[4]  G. Schrauzer Selenomethionine: a review of its nutritional significance, metabolism and toxicity. , 2000, The Journal of nutrition.

[5]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[6]  C. Ghommidh,et al.  Organic acids oxidation by Candida utilis: application to industrial waste water treatment , 1999 .

[7]  A. Pometto,et al.  Enhanced organically bound selenium yeast production by fed-batch fermentation. , 1999, Journal of agricultural and food chemistry.

[8]  E. Woodams,et al.  Utilization of corn silage juice by Klyuveromyces marxianus. , 2003, Bioresource Technology.

[9]  M. Choi,et al.  Growth of Pichia guilliermondii A9, an osmotolerant yeast, in waste brine generated from kimchi production , 1999 .

[10]  K. Nand,et al.  Fungal pretreatment of Orange Processing Waste by solid-state fermentation for improved production of methane , 1995 .

[11]  Geun Eog Ji,et al.  Use of waste Chinese cabbage as a substrate for yeast biomass production. , 2002, Bioresource technology.

[12]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[13]  S. Chanda,et al.  Plant origin liquid waste: A resource for singlecell protein production by yeast , 1996 .

[14]  J. Tay,et al.  Intensive bioconversion of sewage sludge and food waste by Bacillus thermoamylovorans , 2003 .

[15]  J. Rodríguez-Baño,et al.  Nosocomial bacteremia due to Acinetobacter baumannii: epidemiology, clinical features and treatment. , 2002, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[16]  B. Pehrson,et al.  The influence of dietary selenium as selenium yeast or sodium selenite on the concentration of selenium in the milk of Suckler cows and on the selenium status of their calves. , 1999, Journal of animal science.

[17]  I. van Heerden,et al.  Microbial, chemical and physical aspects of citrus waste composting. , 2002, Bioresource technology.

[18]  A. Aro,et al.  Selenium supplementation of children in a selenium-deficient area in China , 2000, Biological Trace Element Research.

[19]  J. Tay,et al.  A hybrid two-phase system for anaerobic digestion of food waste. , 2002, Water science and technology : a journal of the International Association on Water Pollution Research.

[20]  Y. Yun,et al.  Treatment of food wastes using slurry-phase decomposition , 2000 .