[28] Phase determination from multiwavelength anomalous diffraction measurements.

Publisher Summary This chapter discusses phase determination from multiwavelength anomalous diffraction (MAD) measurements. The MAD approach to macromolecular structure determination has potential advantages for accuracy and convenience in phase evaluation. Isomorphism is intrinsically perfect; an algebraically exact analysis is possible; relative scattering strength and phasing power increase with scattering angle; and all required diffraction data can be measured from a single crystal. The virtual immortalization of crystals through freezing makes these advantages real and practical. Although many of the procedures in MAD phasing are in common with crystallographic practice, there are also a number of practical steps unique to this methodology. The chapter discusses the basic foundations of the method, presents the design and execution of experiments, and describes the steps and alternatives for data analysis.

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