Inhibition of human lymphocyte blast transformation by streptolysin O.

Abstract Streptolysin O in the hemolytically active form failed to stimulate in vitro lymphocyte blast transformation as measured by thymidine uptake, while streptolysin O inactivated by heating or cholesterol did stimulate thymidine uptake. Hemolytically active streptolysin O was a very effective inhibitor of phytohemagglutinin-induced lymphocyte transformation, but only when the cells were treated first with streptolysin O. Lymphocytes from a PPD-sensitive donor could not be transformed by PPD when pretreated with streptolysin O. The levels of streptolysin O necessary to block transformation (0.5 to 2.0 HU per milliliter) had no measurable effect on cell viability as measured by Evans-Blue dye uptake and did not cause lysis of lymphocytes.

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