Differential syncytium-inducing capacity of human immunodeficiency virus isolates: frequent detection of syncytium-inducing isolates in patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex

Human immunodeficiency virus isolates were studied with respect to syncytium-inducing capacity, replicative properties, and host range. Five of 10 isolates from patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex were able to induce syncytia in cultures of peripheral blood mononuclear cells (MNC). In contrast, only 2 of 12 isolates from asymptomatic individuals had syncytium-inducing capacity. Syncytium-inducing isolates were reproducibly obtained from the same MNC sample in over 90% of the cases, independent of the donor MNC used for propagation. Syncytium-inducing capacity was shown to be a stable property of an isolate, independent of viral replication rates. Evidence was obtained that the high replication rate of syncytium-inducing isolates observed during primary isolation may be due to higher infectivity of these isolates. The finding that only syncytium-inducing isolates could be transmitted to the H9 cell line is compatible with this higher infectivity. The frequent isolation of syncytium-inducing isolates from individuals with AIDS-related complex or AIDS and the apparent higher in vitro infectivity of these isolates suggest that syncytium-inducing isolates may unfavorably influence the course of human immunodeficiency virus infection.

[1]  J. Levy,et al.  Differential ability of human immunodeficiency virus isolates to productively infect human cells. , 1987, Journal of immunology.

[2]  J. Levy,et al.  CD8+ lymphocytes can control HIV infection in vitro by suppressing virus replication. , 1986, Science.

[3]  J. Mcdougal,et al.  Binding of the human retrovirus HTLV-III/LAV/ARV/HIV to the CD4 (T4) molecule: conformation dependence, epitope mapping, antibody inhibition, and potential for idiotypic mimicry. , 1986, Journal of immunology.

[4]  J. Goudsmit,et al.  PRODUCTIVE HTLV-III INFECTION OF HUMAN B CELL LINES , 1985, The Lancet.

[5]  J. Albert,et al.  REPLICATIVE CAPACITY OF HUMAN IMMUNODEFICIENCY VIRUS FROM PATIENTS WITH VARYING SEVERITY OF HIV INFECTION , 1986, The Lancet.

[6]  J. Levy,et al.  Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions. , 1985, Virology.

[7]  K. Strebel,et al.  The HIV A (sor) gene product is essential for virus infectivity , 1987, Nature.

[8]  S. Wain-Hobson,et al.  Genetic variability of the AIDS virus: Nucleotide sequence analysis of two isolates from African patients , 1986, Cell.

[9]  J. Sodroski,et al.  Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. , 1987, Science.

[10]  W. Darrow,et al.  The acquired immunodeficiency syndrome in a cohort of homosexual men. A six-year follow-up study. , 1985, Annals of internal medicine.

[11]  J. Sodroski,et al.  Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicity , 1986, Nature.

[12]  K. Sell,et al.  Susceptibility of normal human lymphocytes to infection with HTLV-III/LAV. , 1986, Journal of immunology.

[13]  M. Gonda,et al.  Genomic diversity of the acquired immune deficiency syndrome virus HTLV-III: different viruses exhibit greatest divergence in their envelope genes. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Greaves,et al.  The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus , 1984, Nature.

[15]  J K Nicholson,et al.  Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule. , 1986, Science.

[16]  T. A. Hagan,et al.  Detection , Isolation , and Continuous Production of Cytopathic Retroviruses ( HTLV-III ) from Patients with AIDS and Pre-AIDS , 2022 .

[17]  K. Steimer,et al.  Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoprotein , 1986, Nature.

[18]  T. Quinn,et al.  Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire. , 1985, Science.