Rapid response of hepatocyte growth factor in pulmonary ischemia in a rat model.

Hepatocyte growth factor (HGF) is a multifunctional factor implicated in tissue regeneration, wound healing, and angiogenesis. HGF was initially thought to be liver-specific, but it has become clear that HGF acts on alveolar type II cells and bronchial epithelial cells. This study was conducted to determine the role of HGF in pulmonary ischemia in a rat model. The first increase of the plasma HGF level was noted 30 min after pulmonary ischemia, and reached a peak at 12 h. Real-time reverse transcription polymerase chain reaction (Real-time RT-PCR) revealed that the HGF messenger RNA (mRNA) expression in the injured left lung was markedly increased at 1, 6, and 12 h after pulmonary ischemia (P < 0.05). The interleukin-1beta (IL-1beta) mRNA expression, one of the inflammatory cytokines which induces HGF expression, was markedly increased at 1 h in the injured left lung (P = 0.0007). Therefore, we considered that HGF might be mainly induced by paracrine mechanisms in pulmonary ischemia. In conclusion, we have shown that the expression of HGF was induced in pulmonary ischemia, and may be a useful biological marker for the early diagnosis.

[1]  植村 忠廣.  Different expression of positive and negative regulators of hepatocyte growth in growing and shrinking hepatic lobes after portal vein branch ligation in rats , 2000 .

[2]  T. Tsuji,et al.  Different expression of positive and negative regulators of hepatocyte growth in growing and shrinking hepatic lobes after portal vein branch ligation in rats. , 2000, International journal of molecular medicine.

[3]  K. Sugahara,et al.  Elevation of serum human hepatocyte growth factor (HGF) level in patients with pneumonectomy during a perioperative period , 1998, Intensive Care Medicine.

[4]  A. Matsumori,et al.  Enhanced expression of hepatocyte growth factor/c-Met by myocardial ischemia and reperfusion in a rat model. , 1997, Circulation.

[5]  KohOno,et al.  Enhanced Expression of Hepatocyte Growth Factor/c-Met by Myocardial Ischemia and Reperfusion in a Rat Model , 1997 .

[6]  T. Nakamura,et al.  Hepatocyte growth factor in bronchoalveolar lavage fluids and cells in patients with inflammatory chest diseases of the lower respiratory tract: detection by RIA and in situ hybridization. , 1997, American journal of respiratory cell and molecular biology.

[7]  R. Panos,et al.  Intratracheal administration of hepatocyte growth factor/scatter factor stimulates rat alveolar type II cell proliferation in vivo. , 1996, American journal of respiratory cell and molecular biology.

[8]  K. Matsumoto,et al.  In vivo mitogenic action of HGF on lung epithelial cells: pulmotrophic role in lung regeneration. , 1996, The American journal of physiology.

[9]  Y. Shibayama,et al.  Interruption of pulmonary arterial flow with inadequate ventilation leads to pulmonary infarction , 1996, Virchows Archiv.

[10]  K. Higashino,et al.  Elevated serum hepatocyte growth factor/scatter factor levels in inflammatory lung disease. , 1995, American journal of respiratory and critical care medicine.

[11]  G. Michalopoulos,et al.  Hepatocyte growth factor stimulates DNA synthesis in alveolar epithelial type II cells in vitro. , 1995, American journal of respiratory cell and molecular biology.

[12]  C. Leslie,et al.  Hepatocyte growth factor is a growth factor for rat alveolar type II cells. , 1994, American journal of respiratory cell and molecular biology.

[13]  S. Randell,et al.  Growth and differentiation of tracheal epithelial progenitor cells. , 1994, The American journal of physiology.

[14]  K. Matsumoto,et al.  Hepatocyte growth factor may act as a pulmotrophic factor on lung regeneration after acute lung injury. , 1993, The Journal of biological chemistry.

[15]  J. Catravas,et al.  Tyrosine kinase inhibitors suppress endotoxin- and IL-1 beta-induced NO synthesis in aortic smooth muscle cells. , 1993, The American journal of physiology.

[16]  H. Kleinman,et al.  Scatter factor induces blood vessel formation in vivo. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[17]  K. Matsumoto,et al.  Up-regulation of hepatocyte growth factor gene expression by interleukin-1 in human skin fibroblasts. , 1992, Biochemical and biophysical research communications.

[18]  K. Matsumoto,et al.  Lung may have an endocrine function producing hepatocyte growth factor in response to injury of distal organs. , 1992, Biochemical and biophysical research communications.

[19]  K. Matsumoto,et al.  Renotropic functions of hepatocyte growth factor in renal regeneration after unilateral nephrectomy. , 1991, The Journal of biological chemistry.

[20]  K. Miyazawa,et al.  Structural organization and the transcription initiation site of the human hepatocyte growth factor gene. , 1991, Biochemistry.

[21]  K. Matsumoto,et al.  Marked stimulation of growth and motility of human keratinocytes by hepatocyte growth factor. , 1991, Experimental cell research.

[22]  S. Moncada,et al.  Nitric oxide: physiology, pathophysiology, and pharmacology. , 1991, Pharmacological reviews.

[23]  J. Wain,et al.  Inhaled Nitric Oxide: A Selective Pulmonary Vasodilator Reversing Hypoxic Pulmonary Vasoconstriction , 1991, Circulation.

[24]  T. Nakamura,et al.  Identification and change in the receptor for hepatocyte growth factor in rat liver after partial hepatectomy or induced hepatitis. , 1991, Biochemical and biophysical research communications.

[25]  K. Tashiro,et al.  Molecular cloning and expression of human hepatocyte growth factor , 1989, Nature.

[26]  S. Hirono,et al.  Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure. , 1988, The Journal of clinical investigation.

[27]  T. Nakamura,et al.  Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[28]  M. Tsao,et al.  Factors associated with pulmonary infarction. A discriminant analysis study. , 1985, American journal of clinical pathology.

[29]  T. Nakamura,et al.  Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. , 1984, Biochemical and biophysical research communications.

[30]  S. Moncada,et al.  The biological and pharmacological role of nitric oxide in platelet function. , 1993, Advances in experimental medicine and biology.

[31]  M. Terzaghi-Howe,et al.  Basal cells are the progenitors of primary tracheal epithelial cell cultures. , 1992, Experimental cell research.

[32]  N. Johnson,et al.  Epithelial progenitor cells in the rat trachea. , 1990, American journal of respiratory cell and molecular biology.