Versatile broadband electrode assembly for cell electroporation

In this paper, a versatile electrode assembly for cell electroporation is proposed. For validation of the delivery system, biological cell electroporation experiments using 2.5 ns and 5 ns, 10 MV/m pulsed electric fields have been conducted. Electromagnetic, time domain, and frequency analyses demonstrate the broadband behavior of the delivery system.

[1]  P. Thomas Vernier,et al.  Microchamber Setup Characterization for Nanosecond Pulsed Electric Field Exposure , 2011, IEEE Transactions on Biomedical Engineering.

[2]  Martin A Gundersen,et al.  Nanopore-facilitated, voltage-driven phosphatidylserine translocation in lipid bilayers—in cells and in silico , 2006, Physical biology.

[3]  Caterina Merla,et al.  Characterization of a 50-Ω Exposure Setup for High-Voltage Nanosecond Pulsed Electric Field Bioexperiments , 2011, IEEE Transactions on Biomedical Engineering.

[4]  L. Mir,et al.  Application of electroporation gene therapy: past, current, and future. , 2008, Methods in molecular biology.

[5]  Nikolai Dechev,et al.  Development of an Autonomous Biological Cell Manipulator With Single-Cell Electroporation and Visual Servoing Capabilities , 2009, IEEE Transactions on Biomedical Engineering.

[6]  A. Kuthi,et al.  A linear, single-stage, nanosecond pulse generator for delivering intense electric fields to biological loads , 2009, IEEE Transactions on Dielectrics and Electrical Insulation.

[7]  F. Andre,et al.  Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death. , 2010, Bioelectrochemistry.

[8]  L. Duvillaret,et al.  Simultaneous High Intensity Ultrashort Pulsed Electric Field and Temperature Measurements Using a Unique Electro-Optic Probe , 2012, IEEE Microwave and Wireless Components Letters.

[9]  James C. Weaver,et al.  Electroporation of cells and tissues , 2000 .

[10]  Shu Xiao,et al.  Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses. , 2011, Biochimica et biophysica acta.

[11]  Vincent Couderc,et al.  Kilovolt, Nanosecond, and Picosecond Electric Pulse Shaping by Using Optoelectronic Switching , 2010, IEEE Photonics Technology Letters.

[12]  Owe Orwar,et al.  Single-cell electroporation , 2010, Analytical and bioanalytical chemistry.

[13]  Martin A Gundersen,et al.  Nanoelectropulse-driven membrane perturbation and small molecule permeabilization , 2006, BMC Cell Biology.

[14]  Bennett L Ibey,et al.  Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. , 2009, Biochemical and biophysical research communications.