Genetic Determination of the O Antigens of Salmonella Groups B (4,5,12) and C1 (6,7)

Mäkelä, P. Helena (University of Helsinki, Helsinki, Finland). Genetic determination of the O antigens of Salmonella groups B (4,5,12) and C1 (6,7). J. Bacteriol. 91:1115–1125. 1966.—The genetic determination of the O antigens of Salmonella was studied by Hfr or F′ crosses between strains of groups B (antigens 4,5,12) and C1 (antigens 6,7). The main genetic determinants of the specificities 4 of group B and 7 of group C1 behaved as alleles of one locus, called O or O–4/7. This is probably identical with O–4/9, responsible for the serological difference between groups B and D, and with the “rough” locus rouB. At least parts of the antigens 12 of group B and 62 of group C are determined at the same locus. The gene O–5 is closely linked to O–4/7, both mapping in the approximately 2 minutes distance between his and metG in the order his - O–4/7 - O–5 - metG. In crosses of group B donors with group C1 recipients, a serologically new type, called semirough (SR), appeared in most recombinants that had inherited the O-4 allele of the group B donor. These SR forms are serologically intermediate between smooth and rough forms, showing poor stability in saline but possessing the specificities 4 and 12 and (some of them) 5. On the basis of previous biochemical studies, the hypothesis has been put forward that the side chains of their lipopolysaccharide are much shorter than normal group B side chains, probably containing only one repeating unit per side chain. A gene SR-4 responsible for the elongation of group B side chains beyond the first repeating unit was mapped between gal and try, group B and D bacteria being Sr-4+, and group C being SR-4−.

[1]  K. Nikaido,et al.  Genetic Determination of Enzymes Synthesizing O-Specific Sugars of Salmonella Lipopolysaccharides , 1966, Journal of bacteriology.

[2]  L. Baron,et al.  Genetic Mapping of Vi and Somatic Antigenic Determinants in Salmonella , 1965, Journal of bacteriology.

[3]  P. Robbins,et al.  Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[4]  L. Rothfield,et al.  Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[5]  K. Sanderson,et al.  THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM. , 1965, Genetics.

[6]  K. Nikaido,et al.  BIOSYNTHESIS OF CELL WALL POLYSACCHARIDE IN MUTANT STRAINS OF SALMONELLA. IV. SYNTHESIS OF S-SPECIFIC SIDE-CHAIN. , 1965, Biochemical and biophysical research communications.

[7]  H. Nikaido,et al.  SEMIROUGH STRAINS OF SALMONELLA. , 1965, Proceedings of the National Academy of Sciences of the United States of America.

[8]  K. Nikaido,et al.  Rough Mutants of Salmonella Typhimurium (3) Enzymatic Synthesis of Nucleotide-Sugar Compounds , 1964, Nature.

[9]  B. Stocker,et al.  Rough Mutants of Salmonella Typhimurium (2) Serological and Chemical Investigations , 1964, Nature.

[10]  B. Stocker,et al.  Rough Mutants of Salmonella Typhimurium (1) Genetics , 1964, Nature.

[11]  P. Makela.  HFR males in Salmonella abony. , 1963, Genetics.

[12]  J. Lederberg,et al.  Patterns of sexual recombination in enteric bacteria. , 1962, Genetics.

[13]  P. Robbins,et al.  Studies on the chemical basis of the phage conversion of O-antigens in the E-group Salmonellae. , 1962, Biochemistry.

[14]  B. Stocker,et al.  Colicinogeny and recombination. , 1962, British medical bulletin.

[15]  F. Kauffmann.  Die Bakteriologie der Salmonella-Species , 1961 .

[16]  S. Howarth,et al.  Mutation of Bacteria at High Levels of Survival by Ethyl Methane Sulphonate , 1959, Nature.

[17]  J. Lederberg,et al.  Transduction of flagellar characters in Salmonella. , 1953, Journal of general microbiology.

[18]  J. Lederberg,et al.  GENETIC EXCHANGE IN SALMONELLA , 1952, Journal of bacteriology.

[19]  B. Makela.  Inheritance of the 0 Antigens of Salmonella Groups B and D , 2022 .