Cell cycle model for antibody production kinetics.

The specific antibody production rate of hybridoma cells often increases as the specific growth rate of cells decreases. The purpose of this communication is to propose a cell cycle model which may explain these experimental results and to provide direction for the exploration of culture conditions that enhance specific antibody production rate

[1]  J. Gray,et al.  Techniques in Cell Cycle Analysis , 1987, Humana Press.

[2]  J. Weltman,et al.  Synthesis and secretion of light-chain immunoglobulin in two successive cycles of synchronized plasmacytoma cells , 1976, The Journal of cell biology.

[3]  Z. Darżynkiewicz Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry , 1987 .

[4]  N. Byars,et al.  Programmed Synthesis and Export of Immunoglobulin by Synchronized Myeloma Cells , 1970, Nature.

[5]  R. Tobey,et al.  Effects of isoleucine deficiency on nucleic acid and protein metabolism in cultured Chinese hamster cells. Continued ribonucleic acid and protein synthesis in the absence of deoxyribonucleic acid synthesis. , 1972, Biochemistry.

[6]  R. Tobey,et al.  Isoleucine-mediated regulation of genome repliction in various mammalian cell lines. , 1971, Cancer research.

[7]  G. Moore,et al.  Immunoglobulin production in synchronized cultures of human hematopoietic cell lines. I. Variation of cellular immunoglobulin level with the generation cycle. , 1969, Journal of immunology.

[8]  S. Reuveny,et al.  Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors , 1986 .

[9]  J. Mitchison,et al.  The biology of the cell cycle , 1971 .

[10]  J. Fahey,et al.  Limited Periods of Gene Expression in Immunoglobulin-Synthesizing Cells , 1969, Science.

[11]  Studzinski Gp,et al.  Recovery from prolonged ungalances growth induced in HeLa cells by high concentrations of thymidine. , 1967 .