Studies on the ribonucleic Acid from wheat leaves and chloroplasts.

The chloroplast, leaf ribosomal, and leaf total RNA from seedlings of 2 varieties of Triticum durum and 3 varieties of Triticum vulgare were compared. For a given RNA preparation the major nucleotide composition was the same for all varieties. Irrespective of the variety, the chloroplast total RNA had a higher cytidylic and a lower adenylic acid content than the leaf ribosomal RNA, whereas, the guanylic and uridylic acid content of all RNA preparations was essentially the same. Pseudouridylic acid was present in all RNA preparations and was consistently higher in the durum than the vulgare varieties for leaf ribosomal RNA and leaf total RNA. The leaf ribosomal RNA of all varieties had 2 sub-units with sedimentation coefficients of approximately 18 S and 25 S. The molecular weight of the faster sedimenting subunit was about 2 times that of the slower sedimenting subunit.

[1]  D. Spencer,et al.  The nature of the ribonucleic acid of isolated chloroplasts. , 1966, Archives of biochemistry and biophysics.

[2]  R. E. Click,et al.  The isolation of ribonucleic acid from plant, bacterial or animal cells. , 1966, Biochimica et biophysica acta.

[3]  C. J. Pollard,et al.  Ribosomal ribonucleic acids of chloroplastic and mitochondrial preparations. , 1966, Plant physiology.

[4]  D. Hayes,et al.  Association of rapidly labelled bacterial RNA with ribosomal RNA in solutions of high ionic strength. II. , 1966, Journal of molecular biology.

[5]  A. Jagendorf,et al.  Amino Acid incorporation by wheat chloroplasts. , 1966, Plant physiology.

[6]  D. Spencer.  Protein synthesis by isolated spinach chloroplasts. , 1965, Archives of biochemistry and biophysics.

[7]  L. Bogorad,et al.  NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARD , 1965, The Journal of cell biology.

[8]  B. Lane,et al.  The Alkali-stable Trinucleotide Sequences and the Chain Termini in 18S + 28S Ribonucleates from Wheat Germ* , 1965 .

[9]  G. Brawerman,et al.  TEMPLATE AND RIBOSOMAL RIBONUCLEIC ACIDS ASSOCIATED WITH THE CHLOROPLASTS AND THE CYTOPLASM OF EUGLENA GRACILIS. , 1964, Journal of molecular biology.

[10]  D. Spencer,et al.  THE INCORPORATION OF AMINO ACIDS INTO PROTEIN BY CELL-FREE EXTRACTS FROM TOBACCO LEAVES. , 1964, Biochemistry.

[11]  T. R. Richmond,et al.  Aspects of Nucleic Acid Composition in Gossypium. , 1964, Plant physiology.

[12]  D. Glitz,et al.  THE PURIFICATION AND PROPERTIES OF RIBONUCLEIC ACID FROM WHEAT GERM. , 1963, Biochemistry.

[13]  R. Sager,et al.  CHLOROPLAST DNA IN CHLAMYDOMONAS. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[14]  A. Jacobson,et al.  CYTOCHEMICAL STUDIES CONCERNING THE OCCURRENCE AND DISTRIBUTION OF RNA IN PLASTIDS OF ZEA MAYS , 1963, The Journal of cell biology.

[15]  F. Zscheile,et al.  A Chromatographic-Spectrophotometric Method for Determining Nucleotide Composition of RNA in Plants. , 1963, Plant physiology.

[16]  Spirin As.  The "temperature effect" and macromolecular structure of high-polymer ribonucleic acids of various origin. , 1961 .

[17]  A. Spirin.  The "temperature effect" and macromolecular structure of high-polymer ribonucleic acids of various origin. , 1961, Biokhimiia.

[18]  S. Osawa,et al.  Occurrence of the fifth nucleotide in soluble ribonucleic acid in yeast. , 1959, Biochimica et biophysica acta.

[19]  F. Allen,et al.  Terminal components of ribonucleic acids. , 1958, Archives of biochemistry and biophysics.

[20]  F. Davis,et al.  Ribonucleic acids from yeast which contain a fifth nucleotide. , 1957, The Journal of biological chemistry.

[21]  H. Fraenkel-conrat,et al.  Esterification of proteins with alcohols of low molecular weight. , 1945, The Journal of biological chemistry.