论文信息 - DNA probe for Aeromonas salmonicida

DNA probe for Aeromonas salmonicida

A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.

[1] F. Gannon,et al. Characterization of Aeromonas salmonicida strains using DNA probe technology , 1989 .

[2] G. A. Martin,et al. Molecular cloning of two types of GAP complementary DNA from human placenta. , 1988, Science.

[3] T. Trust,et al. DNA:DNA reassociation analysis of Aeromonas salmonicida. , 1988, Journal of general microbiology.

[4] K. Mullis,et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. , 1988, Science.

[5] B. Austin,et al. Bacterial fish pathogens: disease in farmed and wild fish. , 1999 .

[6] C. Yanisch-Perron,et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. , 1985, Gene.

[7] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .

[8] J. H. Crosa,et al. Deoxyribonucleic acid relationships among members of the genus Aeromonas. , 1979, Canadian journal of microbiology.

[9] F. Sanger,et al. DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[10] J. M. Shewan,et al. Some ecological aspects of the bacterial fish pathogen - Aeromonas salmonicida. , 1977 .