Phototransduction in vertebrate rods: An example of the interaction of theory and experiment in neuroscience

A theoretical and experimental study of the response waveforms of vertebrate photoreceptors is presented. A model is described for the physical-chemical processes of light absorption, for the transconduction of the stimulus to a neural signal, and for the cellular system subserving these processes. Experimental tests are made of some of the theoretical implications and are shown to be consistent, one with the other. The role of the sodium pump in shaping the response waveform is considered, and the effects of light adaptation on response amplitude and kinetics are analyzed in terms of the internal transmitter hypothesis. Theory and experiment reveal gaps in one's knowledge and point to needed future investigations.

[1]  D. Baylor,et al.  Light-induced fluctuations in membrane current of single toad rod outer segments , 1977, Nature.

[2]  A. Lee,et al.  [3H]ouabain autoradiography of frog retina , 1980, The Journal of cell biology.

[3]  G. Fain,et al.  Contribution of a caesium-sensitive conductance increase to the rod photoresponse , 1978, Nature.

[4]  M. Piccolino,et al.  Synaptic Transmission between Photoreceptors and Horizontal Cells in the Turtle Retina , 1974, Science.

[5]  W. A. Hagins,et al.  Signal Transmission along Retinal Rods and the Origin of the Electroretinographic a-Wave , 1969, Nature.

[6]  T. Goldsmith,et al.  Effects of Cardiac Glycosides on Electrical Activity in the Isolated Retina of the Frog , 1967, The Journal of general physiology.

[7]  A. Bortoff,et al.  An electrical model of the vertebrate photoreceptor cell. , 1967, Vision research.

[8]  W. A. Hagins.  The visual process: Excitatory mechanisms in the primary receptor cells. , 1972, Annual review of biophysics and bioengineering.

[9]  E. A. Schwartz,et al.  A voltage‐clamp study of the light response in solitary rods of the tiger salamander. , 1979, The Journal of physiology.

[10]  D. Baylor,et al.  Electrical responses of single cones in the retina of the turtle , 1970, The Journal of physiology.

[11]  W. Lederer,et al.  Characterization of the electrogenic sodium pump in cardiac Purkinje fibres , 1980, The Journal of physiology.

[12]  A. Kaneko,et al.  Effects of external ions on the synaptic transmission from photorecptors to horizontal cells in the carp retina. , 1975, The Journal of physiology.

[13]  I. Trifonov.  [Study of synaptic transmission between photoreceptor and horizontal cell by electric stimulations of the retina]. , 1968, Biofizika.

[14]  J E Lisman,et al.  Membrane conductances of photoreceptors. , 1981, Progress in biophysics and molecular biology.

[15]  F. Werblin.  Time‐ and voltage‐dependent ionic components of the rod response. , 1979, The Journal of physiology.

[16]  W. A. Hagins,et al.  Dark current and photocurrent in retinal rods. , 1970, Biophysical journal.

[17]  R. Cone,et al.  Dark Ionic Flux and the Effects of Light in Isolated Rod Outer Segments , 1972, The Journal of general physiology.

[18]  R. Stickgold,et al.  Synaptic excitation and inhibition resulting from direct action of acetylcholine on two types of chemoreceptors on individual amphibian parasympathetic neurones , 1977, The Journal of physiology.

[19]  G. Arden.  Voltage gradients across the receptor layer of the isolated rat retina , 1976, The Journal of physiology.

[20]  W. Hubbell,et al.  Visual transduction in vertebrate photoreceptors. , 1979, Annual review of neuroscience.

[21]  J. Toyoda,et al.  Light-induced resistance changes in single photoreceptors of Necturus and Gekko. , 1969, Vision research.

[22]  P. Witkovsky,et al.  Excitation and adaptation in the vertebrate retina. , 1980, Current topics in eye research.

[23]  T. Tomita.  Electrophysiological study of the mechanisms subserving color coding in the fish retina. , 1965, Cold Spring Harbor symposia on quantitative biology.