Xenopus laevis is highly suitable for studying the mechanisms of olfactory reception for water‐soluble odorants and for airborne odorants. However, the functional differences of cells and component protein molecules in the olfactory receptors of Xenopus have remained obscure. In recent studies, the patterns of sugar residues expressed on the cell surface have been utilized to analyze the characteristics of neurons, because the sugar chains in neurons play very important roles in targeting and cell‐to‐cell communication. In this study, we have determined the distribution of sugar residues and glycoproteins in the olfactory receptor organs of Xenopus using lectins as labeling agents, and characterized the receptors of water‐soluble odorants and of airborne odorants. The results of lectin histochemical analysis show distributional differences of GlcNAc, GalNAc and mannose between the middle chamber and the lateral chamber of the main nasal cavity. Furthermore, a 65 kDa glycoprotein containing mannose, GlcNAc and GalNAc was specifically detected in the medial chamber of the main cavity epithelium in receptor organs of airborne odorants by SDS‐PAGE and lectin blotting. The characteristics of the epithelia demonstrated in this study should further our understanding of the functional differences between the receptors of water‐soluble odorants and of airborne odorants at the molecular level. Anat Rec 255:420–427, 1999. © 1999 Wiley‐Liss, Inc.
[1]
H. Breer,et al.
Two classes of olfactory receptors in xenopus laevis
,
1995,
Neuron.
[2]
D. Riddle,et al.
Lectin identification of olfactory receptor neuron subclasses with segregated central projections
,
1993,
The Journal of neuroscience : the official journal of the Society for Neuroscience.
[3]
D. L. Meyer,et al.
Functional subdivisions of the olfactory system correlate with lectin-binding properties inXenopus
,
1991,
Brain Research.
[4]
P. Barber.
Ulex europeus agglutinin I binds exclusively to primary olfactory neurons in the rat nervous system
,
1989,
Neuroscience.
[5]
B. Key,et al.
Soybean agglutinin binding to the olfactory systems of the rat and mouse
,
1986,
Neuroscience Letters.
[6]
B. Key,et al.
Selective binding of soybean agglutinin to the olfactory system of Xenopus
,
1986,
Neuroscience.
[7]
T. Getchell,et al.
Ontogeny of the Secretory Elements in the Vertebrate Olfactory Mucosa
,
1986
.
[8]
R. Estes.
THE ROLE OF THE VOMERONASAL ORGAN IN MAMMALIAN REPRODUCTION
,
1972
.
[9]
P. Graziadei.
The Olfactory Mucosa of Vertebrates
,
1971
.