The potential of human-derived periodontal ligament stem cells to osteogenic differentiation: An In vitro investigation

Background : Periodontal ligament stem cells (PDLSCs) are considered as a type of mesenchymal stem cell that is beneficial target for numerous clinical applications in periodontal tissue regeneration therapy. Materials and Methods : This study examined the effects of dexamethasone (Dex) on human PDLSCs in vitro . PDLSCs obtained from the roots of patient’s teeth were cultured with Dex (0.01 �M), and their proliferation was measured. The osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity and Alizarin Red-S staining for calcium deposition. Results : After the administration of 0.01 �M Dex, the activity of ALP increased significantly. Furthermore, mineralized nodule formation showing the intracellular calcium deposition was significantly higher in the Dex-treated cells than that of the control cells. Conclusion : Collectively, Dex has positive effects on osteogenic differentiation of human PDLSCs in vitro. It is suggested that PDLSCs may serve as a potential material for periodontal tissue regeneration.

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