Effect of growth rate reduction and genetic modifications on acetate accumulation and biomass yields in Escherichia coli.

[1]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[2]  E. Waygood,et al.  The control of pyruvate kinases of Escherichia coli. I. Physicochemical and regulatory properties of the enzyme activated by fructose 1,6-diphosphate. , 1974, The Journal of biological chemistry.

[3]  David F. Ollis,et al.  Biochemical Engineering Fundamentals , 1976 .

[4]  H. Kornberg,et al.  The enzymic interconversion of acetate and acetyl-coenzyme A in Escherichia coli. , 1977, Journal of general microbiology.

[5]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[6]  Armin Fiechter,et al.  Acetate formation in continuous culture of Escherichia coli K12 D1 on defined and complex media , 1984 .

[7]  W. Holms,et al.  The central metabolic pathways of Escherichia coli: relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate. , 1986, Current topics in cellular regulation.

[8]  W. Holms,et al.  Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. , 1989, Journal of general microbiology.

[9]  M. Domach,et al.  Simple constrained‐optimization view of acetate overflow in E. coli , 1990, Biotechnology and bioengineering.

[10]  G W Luli,et al.  Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations , 1990, Applied and environmental microbiology.

[11]  J. Neway,et al.  Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant , 1990, Applied and environmental microbiology.

[12]  G W Luli,et al.  Glucose-stat, a glucose-controlled continuous culture , 1991, Applied and environmental microbiology.

[13]  J. Bailey,et al.  Influence of expression of the pet operon on intracellular metabolic fluxes of Escherichia coli , 1992, Biotechnology and bioengineering.

[14]  H. Blanch,et al.  Recombinant Protein Expression in High Cell Density Fed-Batch Cultures of Escherichia Coli , 1992, Bio/Technology.

[15]  H C Lim,et al.  Acetic acid formation in escherichia coli fermentation , 1992, Biotechnology and bioengineering.

[16]  G R Jacobson,et al.  Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. , 1993, Microbiological reviews.

[17]  J. Liao,et al.  Alteration of the Biochemical Valves in the Central Metabolism of Escherichia coli a , 1994, Annals of the New York Academy of Sciences.

[18]  Strategies in High‐Level Expression of Recombinant Protein in Escherichia coli , 1994, Annals of the New York Academy of Sciences.

[19]  G. Bennett,et al.  Modification of central metabolic pathway in escherichia coli to reduce acetate accumulation by heterologous expression of the bacillus subtilis acetolactate synthase gene , 1994, Biotechnology and bioengineering.

[20]  G. Bennett,et al.  Effect of modified glucose uptake using genetic engineering techniques on high‐level recombinant protein production in escherichia coli dense cultures , 1994, Biotechnology and bioengineering.

[21]  W R Strohl,et al.  Acetate metabolism by Escherichia coli in high-cell-density fermentation , 1994, Applied and environmental microbiology.

[22]  U. Rinas,et al.  Simple fed-batch technique for high cell density cultivation of Escherichia coli. , 1995, Journal of biotechnology.

[23]  G. Bennett,et al.  Metabolic Engineering of Escherichia coli To Enhance Recombinant Protein Production through Acetate Reduction , 1995, Biotechnology progress.

[24]  A Martinez,et al.  Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis , 1995, Journal of bacteriology.

[25]  F. Bolivar,et al.  Pathway engineering for the production of aromatic compounds in Escherichia coli , 1996, Nature Biotechnology.

[26]  S. Lee,et al.  High cell-density culture of Escherichia coli. , 1996, Trends in biotechnology.

[27]  J. Liao,et al.  Inverse Flux Analysis for Reduction of Acetate Excretion in Escherichia coli , 1997, Biotechnology progress.

[28]  W. R. Farmer,et al.  Reduction of aerobic acetate production by Escherichia coli , 1997, Applied and environmental microbiology.

[29]  F. Bolivar,et al.  Stimulation of glucose catabolism through the pentose pathway by the absence of the two pyruvate kinase isoenzymes in Escherichia coli. , 1998, Biotechnology and bioengineering.

[30]  T. Conway,et al.  What’s for Dinner?: Entner-Doudoroff Metabolism inEscherichia coli , 1998, Journal of bacteriology.