The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.

A complementary DNA (cDNA) for ubiquitin carboxyl-terminal hydrolase isozyme L3 was cloned from human B cells. The cDNA encodes a protein of 230 amino acids with a molecular mass of 26.182 daltons. The human protein is very similar to the bovine homolog, with only three amino acids differing in over 100 residues compared. The amino acid sequence deduced from the cDNA was 54% identical to that of the neuron-specific protein PGP 9.5. Purification of bovine PGP 9.5 confirmed that it is also a ubiquitin carboxyl-terminal hydrolase. These results suggest that a family of such related proteins exists and that their expression is tissue-specific.

[1]  J. Ridgway,et al.  Cloning and Expression of a Yeast Ubiquitin-Protein Cleaving Activity in Escherichia Coli , 1989, Bio/Technology.

[2]  M. Rechsteiner,et al.  Identification of the long ubiquitin extension as ribosomal protein S27a , 1989, Nature.

[3]  Alexander Varshavsky,et al.  The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis , 1989, Nature.

[4]  K. Wilkinson,et al.  Detection, resolution, and nomenclature of multiple ubiquitin carboxyl-terminal esterases from bovine calf thymus. , 1989, Biochemistry.

[5]  H. Wiśniewski,et al.  An Antigenic Profile of Lewy Bodies: Immunocytochemical Indication for Protein Phosphorylation and Ubiquitination , 1989, Journal of neuropathology and experimental neurology.

[6]  I. Grundke‐Iqbal,et al.  Lewy Bodies Contain Epitopes Both Shared and Distinct from Alzheimer Neurofibrillary Tangles , 1988, Journal of neuropathology and experimental neurology.

[7]  P. Galloway.  Antigenic characteristics of neurofibrillary tangles in progressive supranuclear palsy , 1988, Neuroscience Letters.

[8]  S. Love,et al.  Alz-50, Ubiquitin and Tau Immunoreactivity of Neurofibrillary Tangles, Pick Bodies and Lewy Bodies , 1988, Journal of neuropathology and experimental neurology.

[9]  G. Cole,et al.  Ubiquitin-protein conjugates in Alzheimer's lesions , 1987, Neuroscience Letters.

[10]  M. Reitz,et al.  Long-term inhibition of human T-lymphotropic virus type III/lymphadenopathy-associated virus (human immunodeficiency virus) DNA synthesis and RNA expression in T cells protected by 2',3'-dideoxynucleosides in vitro. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[11]  I. Day,et al.  Molecular cloning of cDNA coding for human PGP 9.5 protein , 1987, FEBS letters.

[12]  K. Wilkinson,et al.  Synthesis and characterization of ubiquitin ethyl ester, a new substrate for ubiquitin carboxyl-terminal hydrolase. , 1986, Biochemistry.

[13]  A. Ullrich,et al.  Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors , 1986, Nature.

[14]  K. Wilkinson,et al.  Tryptic peptide mapping of ubiquitin and derivatives using reverse-phase high performance liquid chromatography. , 1986, Analytical biochemistry.

[15]  I. Weissman,et al.  Expression cloning of a lymphocyte homing receptor cDNA: ubiquitin is the reactive species. , 1986, Science.

[16]  A. Ciechanover,et al.  Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85 , 1984, Cell.

[17]  I. A. Rose,et al.  An enzyme with ubiquitin carboxy-terminal esterase activity from reticulocytes. , 1983, Biochemistry.

[18]  R. Thompson,et al.  Isolation of PGP 9.5, a New Human Neurone‐Specific Protein Detected by High‐Resolution Two‐Dimensional Electrophoresis , 1983, Journal of neurochemistry.

[19]  S. Matsui,et al.  Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[20]  K. Kohn,et al.  Metabolism of ubiquitinated histones. , 1981, The Journal of biological chemistry.

[21]  H. Busch,et al.  Timing of ubiquitin synthesis and conjugation into protein A24 during the HeLa cell cycle. , 1980, Biochemical and biophysical research communications.

[22]  A Ciechanover,et al.  Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis. , 1980, Proceedings of the National Academy of Sciences of the United States of America.