Effect of Donation Time on Platelet Concentrates and Fresh‐Frozen Plasma. An in vitro Study

Abstract. To investigate the effect of donation time on the quality of blood components, we measured the platelet count and pH on platelet concentrates, and the factor V and VIII: C levels and fibrinopeptide A concentration on fresh‐frozen plasma by duration of donation time. Platelet concentrates and fresh‐frozen plasma were classified into three groups according to donation time: group 1, less than 10 min; group 2, 10–15 min, and group 3, longer than 15 min. Mean platelet counts of platelet concentrate were: group 1, 8.6±2.5 (in x 1010), group 2, 8.1±2.6, and group 3, 6.5±3.2 (p<0.05). The same pH was maintained in all three groups. The fibrinopeptide A concentrations in groups 1 and 2 were 24±53 and 169±64 ng/ml, respectively, while in group 3 all were >200 ng/ml, indicating correlation of a higher fibrinopeptide A level with longer donation time. Although a higher fibrinopeptide A level indicated a greater degree of thrombin generation, assays of factors V and VIII: C did not show decreased activity in groups 2 and 3.

[1]  J. D. McDonald,et al.  Determinants of Factor VIII Recovery in Cryoprecipitate , 2003, Transfusion.

[2]  O. Skjønsberg,et al.  Thrombin Generation During Collection and Storage of Blood , 1986, Vox Sanguinis.

[3]  A. Farrugia,et al.  Donation Procedure, Fibrinopeptide A, and Factor VIII , 1984, Vox sanguinis.

[4]  G. Kurt,et al.  FPA Content ‐ a Criterion of Quality for Plasma as Factor VIII Source , 1983, Vox sanguinis.

[5]  M. Blombäck,et al.  Improvement of Plasma Quality as Raw Material for Factor VIII:C Concentrates , 1983, Vox sanguinis.

[6]  K. R. Woods,et al.  What Are the Critical Factors in the Production and Quality Control of Frozen Plasma Intended for Direct Transfusion or for Fractionation to Provide Medically Needed Labile Coagulation Factors? , 1983, Vox sanguinis.

[7]  R. Wensley,et al.  Preparation of Improved Cryoprecipitated Factor VIII Concentrate , 1980 .

[8]  H. Gunson.  Variables Involved in Cryoprecipitate Production and their Effect on Factor VIII Activity , 1979, British journal of haematology.

[9]  L. Hoyer,et al.  Thrombin Activation of Factor VIII , 1978, British journal of haematology.

[10]  J. Hellings,et al.  Contributions to the Optimal Use of Human Blood , 1976, Vox sanguinis.

[11]  V. P. Butler,et al.  The generation of fibrinopeptide A in clinical blood samples: evidence for thrombin activity. , 1976, The Journal of clinical investigation.

[12]  P. J. Hoek,et al.  Contributions to the Optimal Use of Human Blood , 1974, Vox sanguinis.

[13]  H. Brummelhuis,et al.  Contributions to the Optimal Use of Human Blood , 1972, Vox sanguinis.

[14]  R. Hardisty,et al.  A One-stage Factor VIII (Antihaemophilic Globulin) Assay and its Use on Venous and Capillary Plasma , 1962, Thrombosis and Haemostasis.

[15]  村上 省三,et al.  Technical manual : 日本語版 , 1985 .