Biology Lab-on-a Chip for Drug Screening

A microfluidic device was designed and tested for screening anti-inflammatory drug candidates using the cell rolling method. Channels were coated with either E- or P-Selectin (adhesion molecule), and isolated human neutrophils or lymphocytes were allowed to roll on the Selectin coated surface under flow conditions which mimic blood flow in the vascular capillary. Undiluted human blood was successfully tested for cell rolling and inhibition. Anti E- or P- Selectin, Sialyl Lewisx and Fucoidan were tested for the inhibition of cell rolling.