Characterization and mathematical correction of hemolysis interference in selected Hitachi 717 assays.

The effect of hemolysis on several assays performed with the Hitachi 717 was quantified by relating the amount of error to the concentration of hemoglobin. Hemolysis interference was judged clinically significant when analyte concentration varied by > 10% from the initial value. Hemolysis interference was significant for alkaline phosphatase, aspartate aminotransferase, alpha-amylase, bilirubin, creatine kinase, gamma-glutamyltransferase, lactate dehydrogenase, lactate dehydrogenase-1, potassium, and theophylline assays. Error (expressed in absolute terms) was linearly dependent on hemoglobin concentration and independent of the initial analyte concentration in each case, except for bilirubin and theophylline, where multiple regression analysis was required to quantify the effect. Relative error was dependent on the initial analyte concentration in all cases. Correction formulas were calculated from linear regression of absolute error vs hemoglobin concentration. Clinical application of correction formulas and mechanisms of hemolysis interference for each assay are discussed.

[1]  W. G. Brydon,et al.  The effect of haemolysis on the determination of plasma constituents. , 1972, Clinica chimica acta; international journal of clinical chemistry.

[2]  M. Schwartz,et al.  Interferences in diagnostic biochemical procedures. , 1973, Advances in clinical chemistry.

[3]  D. Blank,et al.  A model for assessing interference. , 1987, Clinical chemistry.

[4]  W. T. Caraway.  Chemical and diagnostic specificity of laboratory tests. Effect of hemolysis, lipemia, anticoagulants, medications, contaminants, and other variables. , 1962, American journal of clinical pathology.

[5]  D. Blank,et al.  Hemoglobin interference from in vivo hemolysis. , 1985, Clinical chemistry.

[6]  M. Kroll,et al.  Rationale for Using Multiple Regression Analysis with Complex Interferences , 1992, European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies.

[7]  P. Li,et al.  Mechanism of interference by hemoglobin in the determination of total bilirubin. II. Method of Jendrassik-Grof. , 1980, Clinical chemistry.

[8]  S. Pai,et al.  Effects of Hemolysis on Chemistry Tests , 1991 .

[9]  J. Greenson,et al.  The effect of hemolysis on creatine kinase determination. , 1989, Archives of pathology & laboratory medicine.

[10]  P. Mayne,et al.  The Effect of Haemolysis on the Measurement of Plasma Alkaline Phosphatase Activity , 1987, Annals of clinical biochemistry.

[11]  S. Meites.  Letter: Reproducibly simulating hemolysis, for evaluating its interference with chemical methods. , 1973, Clinical chemistry.

[12]  R. Laessig,et al.  The effects of 0.1 and 1.0 per cent erythrocytes and hemolysis on serum chemistry values. , 1976, American journal of clinical pathology.

[13]  M. Bickel,et al.  Effect of in vitro hemolysis on chemical values for serum. , 1978, Clinical chemistry.

[14]  O Sonntag,et al.  Haemolysis as an interference factor in clinical chemistry. , 1986, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.

[15]  N. Gochman,et al.  Development of an aqueous temperature-indicating technique and its application to clinical laboratory instrumentation. , 1976, Clinical chemistry.

[16]  J. Beilby,et al.  Interference by Haemolysis, Icterus and Lipaemia in Assays on the Beckman Synchron CX5 and Methods for Correction , 1990, Annals of clinical biochemistry.

[17]  E. Bernt,et al.  Creatine kinase in serum: 2. Interference of adenylate kinase with the assay. , 1976, Clinical Chemistry.

[18]  K. Ryder,et al.  Graphical comparisons of interferences in clinical chemistry instrumentation. , 1986, Clinical chemistry.

[19]  A. Mather,et al.  Effects of hemolysis on serum electrolyte values. , 1960, Clinical chemistry.