Antifungal efficacy of chitosan nanoparticles against phytopathogenic fungi and inhibition of zearalenone production by Fusarium graminearum

Chitosan (COS) is a natural safe biopolymer that received great attention in agriculture, food, biomedical, pharmaceutical and environmental industries because their biocompatible, biodegradable, non-toxic and non-allergenic natures. The aims of the current study were to synthesize and characterize chitosan nanoparticles (COS-NPs), to evaluate their antifungal activity against phytopathogenic fungi and inhibition of zearalenone (ZEN) production by Fusarium graminearum. The results revealed that the deacetylation degree of COS was 86.9 0.44 %, the average of molar mass was 171.41 ± 0.29 g/mol, molecular weight was 244 ± 7 kDa and the concentration of free amino groups was 0.05 ± 0.019 mol L-1. COS-NPs showed the nanorod form with rough nature and particle size was around 180 nm. COS-NPs showed an excellent antifungal activity against Alternaria tenuis, Aspergillus niger, A. flavus, Baeuvaria bassiana, Fusarium graminearum, Fusarium oxysporum, Penicillium sp. and Sclerotium rolfsii in dose dependent manner. At a concentration of 800 ppm, it inhibits ZEN production by Fusarium graminearum. It could be concluded that COS-NPs are promise candidate as safe antifungal capable for the prevention of ZEN production.

[1]  B. Rihn,et al.  Chitosan nanoparticles and quercetin modulate gene expression and prevent the genotoxicity of aflatoxin B1 in rat liver , 2015, Toxicology reports.

[2]  B. Rihn,et al.  Possible Synergistic Effect and Antioxidant Properties of Chitosan Nanoparticles and Quercetin against Carbon Tetrachloride-Induce Hepatotoxicity in Rats , 2015 .

[3]  Fei Liu,et al.  Physicochemical and morphological properties of size-controlled chitosan–tripolyphosphate nanoparticles , 2015 .

[4]  H. S. Murali,et al.  Zearalenone induced toxicity in SHSY-5Y cells: The role of oxidative stress evidenced by N-acetyl cysteine. , 2014, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[5]  A. Golshani,et al.  Disruption of protein synthesis as antifungal mode of action by chitosan. , 2013, International journal of food microbiology.

[6]  B. Moerschbacher,et al.  Complexation of copper(II) with chitosan nanogels: toward control of microbial growth. , 2013, Carbohydrate polymers.

[7]  Rajesh Thakur,et al.  An In Vitro Study of The Antifungal Activity of Silver/Chitosan Nanoformulations Against Important Seed Borne Pathogens. , 2012 .

[8]  Haliza Katas,et al.  Antifungal Activity of Chitosan Nanoparticles and Correlation with Their Physical Properties , 2012, International journal of biomaterials.

[9]  H. Ai,et al.  Antioxidant, antifungal and antiviral activities of chitosan from the larvae of housefly, Musca domestica L. , 2012, Food chemistry.

[10]  A. B. Kristoffersen,et al.  Influence of Agronomic and Climatic Factors on Fusarium Infestation and Mycotoxin Contamination of Cereals in Norway , 2012, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[11]  Tao Wang,et al.  Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings , 2012 .

[12]  J. Jung,et al.  Antifungal Effects of Silver Nanoparticles (AgNPs) against Various Plant Pathogenic Fungi , 2012, Mycobiology.

[13]  Pengcheng Li,et al.  Novel thiosemicarbazone chitosan derivatives: Preparation, characterization, and antifungal activity , 2012 .

[14]  Y. Park,et al.  Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. , 2012, International immunopharmacology.

[15]  Jung-Ae Kim,et al.  Protective effect of chitosan oligosaccharides against FcɛRI-mediated RBL-2H3 mast cell activation , 2012 .

[16]  C. Rondeau-Mouro,et al.  Functionalization of chitosan by laccase-catalyzed oxidation of ferulic acid and ethyl ferulate under heterogeneous reaction conditions. , 2012, Carbohydrate polymers.

[17]  P. Dutta,et al.  A new chitosan-thymine conjugate: synthesis, characterization and biological activity. , 2011, International journal of biological macromolecules.

[18]  Shengmin Zhang,et al.  Dual-functional composite with anticoagulant and antibacterial properties based on heparinized silk fibroin and chitosan. , 2011, Colloids and surfaces. B, Biointerfaces.

[19]  M. Badawy,et al.  A Biopolymer Chitosan and Its Derivatives as Promising Antimicrobial Agents against Plant Pathogens and Their Applications in Crop Protection , 2011 .

[20]  S. Nair,et al.  Biomaterials based on chitin and chitosan in wound dressing applications. , 2011, Biotechnology advances.

[21]  Y. Liu,et al.  Preparation and characterization of novel curdlan/chitosan blending membranes for antibacterial applications , 2011 .

[22]  Jie Chen,et al.  Biological activities of chitosan and chitooligosaccharides , 2011 .

[23]  R. Oueslati,et al.  Immunotoxicity of zearalenone in Balb/c mice in a high subchronic dosing study counteracted by Raphanus sativus extract , 2010, Immunopharmacology and immunotoxicology.

[24]  Facundo Ruiz,et al.  Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. , 2010, Nanomedicine : nanotechnology, biology, and medicine.

[25]  N. Magan,et al.  Limiting mycotoxins in stored wheat , 2010, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[26]  N. Read,et al.  Membrane fluidity determines sensitivity of filamentous fungi to chitosan , 2010, Molecular microbiology.

[27]  Xiaoxiao Zhu,et al.  The performance of a fly-larva shell-derived chitosan sponge as an absorbable surgical hemostatic agent. , 2010, Biomaterials.

[28]  K. Nicolay,et al.  Chitosan-based systems for molecular imaging. , 2010, Advanced drug delivery reviews.

[29]  M. Thanou,et al.  Biodegradation, biodistribution and toxicity of chitosan. , 2010, Advanced drug delivery reviews.

[30]  V. Bobbarala,et al.  Antifungal Activity of Selected Plant Extracts against Phytopathogenic Fungi Aspergillus niger F2723 , 2009 .

[31]  M. Jolicoeur,et al.  Fractionation and characterization of chitosan by analytical SEC and 1H NMR after semi-preparative SEC , 2009 .

[32]  Seong-Cheol Park,et al.  Investigation of the antifungal activity and mechanism of action of LMWS-chitosan. , 2008, Journal of microbiology and biotechnology.

[33]  M. Otagiri,et al.  Antioxidant protection of human serum albumin by chitosan. , 2008, International journal of biological macromolecules.

[34]  J. Fink-Gremmels,et al.  In vitro assessment of adsorbents aiming to prevent deoxynivalenol and zearalenone mycotoxicoses , 2007, Mycopathologia.

[35]  Yuan'an Wei,et al.  Determination of the degree of acetylation of chitosan by UV spectrophotometry using dual standards. , 2006, Carbohydrate research.

[36]  Yi Liu,et al.  Water-solubility of chitosan and its antimicrobial activity , 2006 .

[37]  L. Jia.  Nanoparticle Formulation Increases Oral Bioavailability of Poorly Soluble Drugs: Approaches Experimental Evidences and Theory. , 2005, Current nanoscience.

[38]  Gadi Borkow,et al.  Copper as a biocidal tool. , 2005, Current medicinal chemistry.

[39]  Zi-rong Xu,et al.  Preparation and antibacterial activity of chitosan nanoparticles. , 2004, Carbohydrate research.

[40]  김세권,et al.  Potential Immuno-Stimulating Effect of Antitumoral Fraction of Chitosan Oligosaccharides , 2001 .

[41]  G. Giménez-Gallego,et al.  Myo-inositol hexasulphate and low molecular weight heparin binding to human acidic fibroblast growth factor: a calorimetric and FTIR study. , 2001, International journal of biological macromolecules.

[42]  J. Desbrières,et al.  An infrared investigation in relation with chitin and chitosan characterization , 2001 .

[43]  F. Cui,et al.  A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. , 2000, Journal of biomedical materials research.

[44]  T. Miller,et al.  Dietary chitosan inhibits hypercholesterolaemia and atherogenesis in the apolipoprotein E-deficient mouse model of atherosclerosis. , 1998, Atherosclerosis.

[45]  K. Domsch,et al.  Compendium of Soil Fungi , 1995 .

[46]  J. Pitt,et al.  Fungi and Food Spoilage , 1987 .

[47]  L. Hadwiger,et al.  The fungicidal effect of chitosan on fungi of varying cell wall composition , 1979 .

[48]  P. Bragg,et al.  The effect of silver ions on the respiratory chain of Escherichia coli. , 1974, Canadian journal of microbiology.

[49]  D. B. Duncan.  Multiple range tests for correlated heteroscedastic means , 1957 .

[50]  C. Decock,et al.  Compendium of soil fungi, 2nd edition , 2009 .

[51]  Juan Carlos Moltó,et al.  Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: an oestrogenic mycotoxin. , 2007, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[52]  Zhen-Xing Tang,et al.  Preparation of chitosan nanoparticles as carrier for immobilized enzyme , 2007, Applied biochemistry and biotechnology.

[53]  P. Park,et al.  Antimicrobial Activity of Hetero-Chitosans and Their Oligosaccharides with Different Molecular Weights , 2004 .

[54]  S. Bautista-Bañosa,et al.  Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides , anthracnose levels and quality of papaya fruit , 2003 .

[55]  Jaspal Singh,et al.  Inhibition of storage fungi of blackgram (Vigna mungo L.) by some essential oils , 1999 .