The technic for isolation-perfusion of the rat hind limb.

Summary The technic for isolation-perfusion of the rat hind limb is described. By the standardized technic, limb viability was well maintained, and leakage of anticancer agents into the systemic circulation was negligible. The gross and microscopic response of the Walker 256 carcinosarcoma to perfusion with triethylenethiophosphoramide is described. The preparation permits study of the many variables related to perfusion. The technic is adaptable to other small animals.

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