The projection domain of MAP4 suppresses the microtubule-bundling activity of the microtubule-binding domain.

[1]  H. Hotani,et al.  Ser787 in the proline-rich region of human MAP4 is a critical phosphorylation site that reduces its activity to promote tubulin polymerization. , 2000, Cell structure and function.

[2]  S. Kotani,et al.  A new model for microtubule-associated protein (MAP)-induced microtubule assembly. The Pro-rich region of MAP4 promotes nucleation of microtubule assembly in vitro. , 1999, European journal of biochemistry.

[3]  A. Okuyama,et al.  MAP4 is the in vivo substrate for CDC2 kinase in HeLa cells: identification of an M-phase specific and a cell cycle-independent phosphorylation site in MAP4. , 1997, Biochemistry.

[4]  H. Hotani,et al.  Phosphorylation states of microtubule-associated protein 2 (MAP2) determine the regulatory role of MAP2 in microtubule dynamics. , 1997, Biochemistry.

[5]  J. Bulinski,et al.  Overexpression of full- or partial-length MAP4 stabilizes microtubules and alters cell growth. , 1997, Journal of cell science.

[6]  M. Inagaki,et al.  Microinjection of intact MAP-4 and fragments induces changes of the cytoskeleton in PtK2 cells. , 1996, Cell motility and the cytoskeleton.

[7]  J R McIntosh,et al.  Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras , 1995, The Journal of cell biology.

[8]  H. Hotani,et al.  Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics , 1995, The Journal of cell biology.

[9]  E. Mandelkow,et al.  Microtubules and microtubule-associated proteins. , 1995, Current opinion in cell biology.

[10]  A. Matus,et al.  Sequence analysis of MAP2 function in living cells. , 1994, Journal of cell science.

[11]  H. Hotani,et al.  Microtubule-stabilizing activity of microtubule-associated proteins (MAPs) is due to increase in frequency of rescue in dynamic instability: shortening length decreases with binding of MAPs onto microtubules. , 1994, Cell structure and function.

[12]  E. Mandelkow,et al.  Domains of tau protein and interactions with microtubules. , 1994, Biochemistry.

[13]  M. Gonzalez-Garay,et al.  Stable expression of heterologous microtubule-associated proteins (MAPs) in Chinese hamster ovary cells: evidence for differing roles of MAPs in microtubule organization , 1994, The Journal of cell biology.

[14]  A. Matus,et al.  Bundling of microtubules in transfected cells does not involve an autonomous dimerization site on the MAP2 molecule. , 1994, Molecular biology of the cell.

[15]  N. Hirokawa.  Microtubule organization and dynamics dependent on microtubule-associated proteins. , 1994, Current opinion in cell biology.

[16]  N. Hirokawa,et al.  Projection domains of MAP2 and tau determine spacings between microtubules in dendrites and axons , 1992, Nature.

[17]  N. Hirokawa,et al.  Microtubule bundling by tau proteins in vivo: analysis of functional domains. , 1992, The EMBO journal.

[18]  G. Lee,et al.  Expression of tau protein in non-neuronal cells: microtubule binding and stabilization. , 1992, Journal of cell science.

[19]  R. Vallee,et al.  Domain structure and antiparallel dimers of microtubule-associated protein 2 (MAP2). , 1992, Journal of Structural Biology.

[20]  J. Olmsted,et al.  A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences. , 1991, The Journal of biological chemistry.

[21]  K. Suzuki,et al.  Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U). , 1991, The Journal of biological chemistry.

[22]  J. Bulinski,et al.  Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau. , 1991, Journal of cell science.

[23]  C. Garner,et al.  Molecular structure of microtubule-associated protein 2b and 2c from rat brain. , 1990, The Journal of biological chemistry.

[24]  H. Kawasaki,et al.  Molecular cloning of a ubiquitously distributed microtubule-associated protein with Mr 190,000. , 1990, The Journal of biological chemistry.

[25]  I. Ivanov,et al.  Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau , 1989, Nature.

[26]  J. Joly,et al.  The microtubule-binding fragment of microtubule-associated protein-2: location of the protease-accessible site and identification of an assembly-promoting peptide , 1989, The Journal of cell biology.

[27]  N. Hirokawa,et al.  Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA , 1989, The Journal of cell biology.

[28]  S. Lewis,et al.  Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein , 1988, Science.

[29]  J. Walker,et al.  Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein tau. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[30]  M. Kirschner,et al.  The primary structure and heterogeneity of tau protein from mouse brain. , 1988, Science.

[31]  N. Hirokawa,et al.  Limited chymotryptic digestion of bovine adrenal 190,000-Mr microtubule-associated protein and preparation of a 27,000-Mr fragment which stimulates microtubule assembly. , 1987, The Journal of biological chemistry.

[32]  N. Hirokawa,et al.  Purification and characterization of a 190-kD microtubule-associated protein from bovine adrenal cortex , 1986, The Journal of cell biology.

[33]  K. Böhm,et al.  Effect of microtubule-associated proteins on the protofilament number of microtubules assembled in vitro. , 1984, Biochimica et biophysica acta.

[34]  G. Borisy,et al.  Immunofluorescence localization of HeLa cell microtubule-associated proteins on microtubules in vitro and in vivo , 1980, The Journal of cell biology.

[35]  J. Rosenbaum,et al.  The periodic association of MAP2 with brain microtubules in vitro , 1979, The Journal of cell biology.

[36]  G. Borisy,et al.  Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin. , 1977, The Journal of biological chemistry.

[37]  G. Borisy,et al.  Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[38]  M. Kirschner,et al.  A protein factor essential for microtubule assembly. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[39]  C. Cantor,et al.  Microtubule assembly in the absence of added nucleotides. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[40]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.