Comparsions of developmental stages of microspore by bud size and embryogenesis from its microspore in Brassica species

The purpose of this experiment is to search for the identifying method that separate buds with embryogenic potential from collected buds for increasing the efficiency of embryogenesis by microspore culture in Brassica species.In Brassica napus, Lisandra of spring type showed bud-size of 3.2-4.1mm which belongs to late-uninucleate stage as embryogenic. Hallyuchae of winter type showed bud-size of 3.1-3.6mm which belongs to late-uninucleate stage as embryogenic microspores. Two genotypes of B. juncea were nearly same as bud-size of Youngsanpohwanggyeja were 2.9-4.0mm and bud-size of Hwanggyeja were 2.9-4.1mm. Also, two genotypes of B. campestris were nearly same as bud-size of Hyakusai were 2.9-3.4mm and bud-size of Sosongchae were 2.9-3.2mm.In the embryo yield per 100,000 microspores by isolated microspore culture from the buds with embryonic microspores, Youngsanpohwanggyeja of B. juncea were 36 embryos and Hwanggyeja were 19 embryos. B. campestris of Hyakusai were 253 embryos and Sosongchae were 104 embryos. However in B. napus was showed much difference by the growth habits, the spring type of Lisandra were 4,128 embryos of which higher about 8.8 times than those of winter type Hallayuchae.