Guidelines for validation of qualitative real-time PCR methods

As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary. URI: http://www.sciencedirect.com/science/article/pii/S0924224414000661 [1] Authors: BROEDERS Sylvia HUBER Ingrid GROHMANN Lutz BERBEN Gilbert TAVERNIERS Isabel MAZZARA Marco ROOSENS Nancy MORISSET Dany Publication Year: 2014 Type: Articles in Journals Publisher: ELSEVIER SCIENCE LONDON ISSN: 0924-2244 DOI: 10.1016/j.tifs.2014.03.008 [2] Citation: TRENDS IN FOOD SCIENCE \& TECHNOLOGY p. 115-126 no. 2 vol. 37 Source URL: https://ec.europa.eu/jrc/en/publication/articles-journals/guidelines-validation-qualitative-real-time-pcr-methods Links [1] http://www.sciencedirect.com/science/article/pii/S0924224414000661 [2] http://dx.doi.org/10.1016/j.tifs.2014.03.008

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