How to configure a computer-aided neuron reconstruction and graphics display system.

Abstract This paper answers many requests of the authors from scientists who wish to establish computer-aided neuron reconstruction systems. Computer-aided neuron reconstruction is recording of neuron trees in a computer, correcting for histological processing problems, displaying neurons in three dimensions, and statistically summarizing neurons. The system components, a light microscope, a computer, and a graphics display, may be configured in four ways, each having advantages in cost, labor, programming, and maximum neuron complexity and resolution. Software should be written in a high-level language and should be copied from existing installations whenever possible. A neuroscientist may learn the skills necessary to direct, program, and maintain a system, but only at a great cost in time. Alternatively, the selection of a skilled person to perform these tasks critically affects the system's neuroscientific results.

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