Line-scanning fiber bundle endomicroscopy with a virtual detector slit.

Coherent fiber bundles can be used to relay the image plane from the distal tip of an endomicroscope to an external confocal microscopy system. The frame rate is therefore determined by the speed of the microscope's laser scanning system which, at 10-20 Hz, may be undesirably low for in vivo clinical applications. Line-scanning allows an increase in the frame rate by an order of magnitude in exchange for some loss of optical sectioning, but the width of the detector slit cannot easily be adapted to suit different imaging conditions. The rolling shutter of a CMOS camera can be used as a virtual detector slit for a bench-top line-scanning confocal microscope, and here we extend this idea to endomicroscopy. By synchronizing the camera rolling shutter with a scanning laser line we achieve confocal imaging with an electronically variable detector slit. This architecture allows us to acquire every other frame with the detector slit offset by a known distance, and we show that subtracting this second image leads to improved optical sectioning.

[1]  Rebecca Richards-Kortum,et al.  High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging , 2011, Journal of visualized experiments : JoVE.

[2]  Timothy J Muldoon,et al.  Subcellular-resolution molecular imaging within living tissue by fiber microendoscopy. , 2007, Optics express.

[3]  Y. Sabharwal,et al.  Slit-scanning confocal microendoscope for high-resolution in vivo imaging. , 1999, Applied optics.

[4]  Joel N. Bixler,et al.  Confocal Endomicroscopy: Instrumentation and Medical Applications , 2012, Annals of Biomedical Engineering.

[5]  A. Polglase,et al.  Confocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo. , 2004, Gastroenterology.

[6]  Tim N. Ford,et al.  Fluorescence endomicroscopy with structured illumination. , 2008, Optics express.

[7]  Xiao Wang,et al.  Needle-based fluorescence endomicroscopy via structured illumination with a plastic, achromatic objective , 2013, Journal of biomedical optics.

[8]  M. Koucký,et al.  Axial response of high-resolution microendoscopy in scattering media. , 2013, Biomedical optics express.

[9]  Guang-Zhong Yang,et al.  High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking. , 2015, Biomedical optics express.

[10]  G S Kino,et al.  Micromachined scanning confocal optical microscope. , 1996, Optics letters.

[11]  Jerome Mertz,et al.  Fast optically sectioned fluorescence HiLo endomicroscopy. , 2012, Journal of biomedical optics.

[12]  Kung-Bin Sung,et al.  Fiber optic confocal reflectance microscopy: a new real-time technique to view nuclear morphology in cervical squamous epithelium in vivo. , 2003, Optics express.

[13]  A. Polglase,et al.  A fluorescence confocal endomicroscope for in vivo microscopy of the upper- and the lower-GI tract. , 2005, Gastrointestinal endoscopy.

[14]  A. Rouse,et al.  Multispectral imaging with a confocal microendoscope. , 2000, Optics letters.

[15]  Nicholas Ayache,et al.  Towards Optical Biopsies with an Integrated Fibered Confocal Fluorescence Microscope , 2004, MICCAI.

[16]  Improved sectioning in a slit scanning confocal microscope. , 2008, Optics letters.

[17]  Sharmila Anandasabapathy,et al.  Low-Cost Endomicroscopy in the Esophagus and Colon , 2011, The American Journal of Gastroenterology.

[18]  A. Gmitro,et al.  Confocal microscopy through a fiber-optic imaging bundle. , 1993, Optics letters.

[19]  P. Fomitchov,et al.  A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture , 2012, Journal of microscopy.

[20]  Guang-Zhong Yang,et al.  Force adaptive robotically assisted endomicroscopy for intraoperative tumour identification , 2015, International Journal of Computer Assisted Radiology and Surgery.

[21]  Nils Norlin,et al.  Confocal multiview light-sheet microscopy , 2015, Nature Communications.

[22]  Ulrich Kubitscheck,et al.  Scanned light sheet microscopy with confocal slit detection. , 2012, Optics Express.

[23]  Alexander Meining,et al.  A pilot study of in vivo identification of pancreatic cystic neoplasms with needle-based confocal laser endomicroscopy under endosonographic guidance , 2013, Endoscopy.

[24]  A. Rouse,et al.  Clinical confocal microlaparoscope for real-time in vivo optical biopsies. , 2009, Journal of biomedical optics.

[25]  Alexandros D. Polydorides,et al.  Discrimination of Benign and Neoplastic Mucosa with a High-Resolution Microendoscope (HRME) in Head and Neck Cancer , 2012, Annals of Surgical Oncology.

[26]  A. Rouse,et al.  Multispectral confocal microendoscope for in vivo and in situ imaging. , 2008, Journal of biomedical optics.

[27]  Rebecca Richards-Kortum,et al.  A Pilot Study of Low-Cost, High-Resolution Microendoscopy as a Tool for Identifying Women with Cervical Precancer , 2012, Cancer Prevention Research.

[28]  R. Richards-Kortum,et al.  Optimizing modulation frequency for structured illumination in a fiber-optic microendoscope to image nuclear morphometry in columnar epithelium. , 2015, Biomedical optics express.

[29]  Sharmila Anandasabapathy,et al.  In vivo endomicroscopy improves detection of Barrett's esophagus-related neoplasia: a multicenter international randomized controlled trial (with video). , 2014, Gastrointestinal endoscopy.

[30]  Thomas D. Wang,et al.  In vivo near-infrared dual-axis confocal microendoscopy in the human lower gastrointestinal tract. , 2012, Journal of biomedical optics.