Protective effect of prostaglandin E1 (PGE1) on energy metabolism and reticuloendothelial function in the ischemically damaged canine liver.

The protective effects of PGE1 on ischemia-related liver damage were evaluated in dogs. Ninety minutes warm hepatic ischemia was induced by the total clamping of hepatic inflow vasculatures with portal bypassing. The survival rate improved up to 62.5% when PGE1 was administered intravenously prior to ischemia, while no dog survived for longer than 1 week in the nontreated group. Hepatic ATP content was restored up to 80% of preischemic level 2 h after reflow in the PGE1 pretreated group, compared to 55% recovery in the nontreated group. Complete normalization of hepatic energy charge and rapid decrease of lactate were also seen in the PGE1 group. The clearance rate of intravascular lipid emulsion remained fairly normal in the PGE1 group, thereby suggesting well-preserved hepatic reticuloendothelial functions. The serum activities of beta-glucuronidase, GOT and GPT were suppressed in the PGE1-pretreated group, thereby implying a well-protected hepatic integrity. The histology revealed well-preserved hepatic architecture. The remarkable cytoprotective effect of PGE1 on hepatic ischemia shown in this study indicates that PGE1 warrants further study for protection of ischemically compromised hepatic allografts.

[1]  C. Valeri,et al.  Vasodilator prostaglandins (PG) prevent renal damage after ischemia. , 1987, Annals of surgery.

[2]  T. Tsumagari,et al.  Protective effect of prostaglandin E1 on ischemia-induced acute renal failure in dogs. , 1985, Surgery.

[3]  G. Otto,et al.  Preservation damage in liver transplantation: electron-microscopic findings. , 1984, Transplantation proceedings.

[4]  D. V. van Thiel,et al.  Liver Procurement for Orthotopic Transplantation: An Analysis of the Pittsburgh Experience , 1984, Hepatology.

[5]  W. Jubiz,et al.  Dissociation of E prostaglandin effects on liver glycogenolysis and cyclic AMP levels , 1983, Molecular and Cellular Endocrinology.

[6]  M. Monden,et al.  Cytoprotective effect of prostaglandin I2 on ischemia-induced hepatic cell injury. , 1983, Transplantation.

[7]  M. Monden,et al.  Twenty‐four- and 48‐hour Canine Liver Preservation by Simple Hypothermia with Prostacyclin , 1982, Annals of surgery.

[8]  K. Ozawa,et al.  Significance of mitochondrial enhancement in restoring hepatic energy charge after revascularization of isolated ischemic liver. , 1982, Transplantation.

[9]  R. T. Jones,et al.  The pathophysiology of septic shock: studies of reticuloendothelial system function and liver high-energy metabolism in rats following sublethal and lethal Escherichia coli injection. , 1982, Advances in shock research.

[10]  I. Chaudry,et al.  Alterations in cell function with ischemia and shock and their correction. , 1981, Archives of surgery.

[11]  J. Nolan.  Endotoxin, reticuloendothelial function, and liver injury , 1981, Hepatology.

[12]  A. M. Lefer,et al.  Cytoprotective actions of prostacyclin during hypoxia in the isolated perfused cat liver. , 1980, The American journal of physiology.

[13]  S. Powers,et al.  Human host defense mechanisms as they relate to surgery and trauma. , 1980, Surgery annual.

[14]  G. Machiedo,et al.  Comparison of corticosteroids and prostaglandins in treatment of hemorrhagic shock. , 1979, Annals of surgery.

[15]  J. Farber,et al.  Accelerated phospholipid degradation and associated membrane dysfunction in irreversible, ischemic liver cell injury. , 1978, The Journal of biological chemistry.

[16]  D. K. Kim,et al.  Measurement of phagocytic activity of reticuloendothelial system (RES) by Intralipid: effect of C parvum treatment. , 1977, Surgical forum.

[17]  D. Loegering,et al.  Correlation of Plasma Lysosomal Enzyme Levels with Hepatic Reticuloendothelial Function after Trauma 1 , 1976, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[18]  F. DeRubertis,et al.  Effects of prostaglandins on hepatic adenylate cyclase activity and cyclic adenosine 3',5',-monophosphate content. , 1974, Endocrinology.

[19]  S. Fleischer,et al.  Characterization and localization of prostaglandin E1 receptors in rat liver plasma membranes , 1974 .

[20]  A. M. Lefer,et al.  Mechanism of the protective effects of prostaglandins E1 and F2α in canine endotoxin shock , 1973 .

[21]  J. Starling,et al.  Protective Effect of Prostaglandin E1 (PGE1) on Lysosomal Enzyme Release in Serotonin‐Induced Gastric Ulceration , 1973, Annals of surgery.

[22]  L. Ignarro,et al.  Effects of prostaglandins on release of enzymes from lysosomes of pancreas, spleen and kidney cortex , 1973 .

[23]  A. M. Lefer,et al.  Circulatory Responses to Splanchnic Lysosomal Hydrolases in the Dog , 1972, Annals of surgery.

[24]  L. Maclean,et al.  Predictive value of liver biochemistry in acute hepatic ischemia. , 1971, Surgery, gynecology & obstetrics.

[25]  T. Inouye,et al.  Immediate and long-term effects of acute hepatic ischemia. , 1967, Archives of surgery.

[26]  Keitaro Kato,et al.  Synthesisof p-Nitrophenyl β-D-Glucopyranosiduronic Acid and Its Utilization as a Substrate for the Assay of β-Glucuronidase Activity , 1960 .