Morphological characteristics and cell viability of coffee plants calli

O objetivo deste trabalho foi caracterizar e comparar dois tipos de calos de explantes foliares de Coffea arabica (cultivar Catigua). Celulas de diferentes tipos de calos foram caracterizadas quanto a viabilidade e caracteristicas morfologicas externas e internas. Foram obtidos dois tipos de calos morfologicamente distintos: (a) amarelo friavel e (b) transparente aquoso. Os calos amarelos friaveis apresentaram maior viabilidade celular e caracteristicas embriogenicas. Microscopia eletronica de varredura e transmissao mostraram caracteristicas embriogenicas em calos amarelos friaveis evidenciadas pela presenca de celulas pequenas e isodiametricas. Os calos transparentes aquosos apresentaram celulas alongadas e vacuolizadas.

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