Limonoid Debittering of Citrus Juice Sera By Immobilized Cells of Corynebacterium fascians

Corynebacterium fascians cells capable of metabolizing limonoids were prepared conveniently with inexpensive carbon sources such as fructose, galactose and citric acid. Cells thus obtained were immo-bilized in acrylamide gel and used in a biological debittering process previously developed. The process significantly reduced limonin and nomilin contents of citrus juice sera. It was particularly effective toward the reduction of nomilin. The debittering treatment did not have adverse effects on the composition of other citrus constituents such as citric, malic, ascorbic acids, fructose, glucose and sucrose.

[1]  S. Hasegawa,et al.  Nomilin Acetyl-Lyase, a Bacterial Enzyme for Nomilin Debittering of Citrus Juices , 2006 .

[2]  S. Hasegawa,et al.  Metabolism of limonoids: conversion of nomilin to obacunone in Corynebacterium fascians , 1984 .

[3]  S. Hasegawa,et al.  Metabolism of limonoids by Arthrobacter globiformis. II. Basis for a practical means of reducing the limonin content of orange juice by immobilized cells. , 1983 .

[4]  A. King,et al.  A species of bacterium-producing constitutive enzymes for limonoid metabolism , 1983 .

[5]  S. Hasegawa,et al.  Metabolism of limonoate via a trans-19-hydroxyobacunoate pathway , 1983 .

[6]  Mayur Patel,et al.  Reduction of limonin bitterness in navel orange juice serum with bacterial cells immobilized in acrylamide gel , 1982 .

[7]  S. Hasegawa,et al.  Metabolism of limonoids. Isolation and characterization of deoxylimonin hydrolase from Pseudomonas. , 1974, Journal of agricultural and food chemistry.

[8]  T. Tosa,et al.  Basic studies for continuous production of L-aspartic acid by immobilized Escherichia coli cells. , 1974, Applied microbiology.

[9]  A. King,et al.  Isolation of new limonoate dehydrogenase from Pseudomonas. , 1974, Journal of agricultural and food chemistry.

[10]  A. King,et al.  Limonoate dehydrogenase from Arthrobacter globiformis. , 1972, Journal of agricultural and food chemistry.

[11]  E. Grant,et al.  Specific thin-layer chromatography assay of limonin, a citrus bitter principle , 1970 .

[12]  S. Hasegawa,et al.  Reduction of nomilin bitterness in citrus juices and juice serum with Arthrobacter globiformis cells immobilized in acrylamide gel , 1983 .