Immobilization of cellulase in nanofibrous PVA membranes by electrospinning

Electrospinning is a nanofiber-forming process by which either polymer solution or melt is charged to high voltages. With high specific surface area and porous structure, electrospun fibrous membranes are excellent candidates for immobilization of enzymes. In this paper, immobilization of cellulase in nanofibrous poly(vinyl alcohol) (PVA) membranes was studied by electrospinning. PVA and cellulase were dissolved together in an acetic acid buffer (pH 4.6) and electrospun into nanofibers with diameter of around 200 nm. The nanofibrous membranes were crosslinked by glutaraldehyde vapor and examined catalytic efficiency for biotransformations. The activity of immobilized cellulase in PVA nanofibers was over 65% of that of the free enzyme. Nanofibers were superior to casting films from the same solution for immobilization of cellulase. The activity of immobilized cellulase descended with ascending in enzyme loading efficiency and crosslinking time, which retained 36% its initial activity after six cycles of reuse.

[1]  Ping Wang,et al.  Enzyme‐Carrying Polymeric Nanofibers Prepared via Electrospinning for Use as Unique Biocatalysts , 2002, Biotechnology progress.

[2]  T. I. Velichko,et al.  Activity and Stability of Native and Modified Subtilisins in Various Media , 2003, Biochemistry (Moscow).

[3]  Eyal Zussman,et al.  Experimental investigation of the governing parameters in the electrospinning of polymer solutions , 2004 .

[4]  Xiaoyan Yuan,et al.  Immobilization of cellulase using acrylamide grafted acrylonitrile copolymer membranes , 1999 .

[5]  Yongcheng Liu,et al.  Immobilization of glucose oxidase onto the blend membrane of poly(vinyl alcohol) and regenerated silk fibroin: morphology and application to glucose biosensor , 1996 .

[6]  Seçil Önal,et al.  Preparation and Properties of α‐Galactosidase Chemically Attached to Activated Chitin , 2003 .

[7]  T. K. Ghose.  Measurement of cellulase activities , 1987 .

[8]  R. Tan,et al.  Immobilization of glucose oxidase on chitosan–SiO2 gel , 2004 .

[9]  K. Storey,et al.  Immobilization of cellulase using polyurethane foam , 1988 .

[10]  You-Lo Hsieh,et al.  Ultra-high surface fibrous membranes from electrospinning of natural proteins: casein and lipase enzyme , 2003 .

[11]  R. Farris,et al.  Mechanical behavior of electrospun polyurethane , 2003 .

[12]  F. Plieva,et al.  Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments , 1998 .

[13]  S. Shivkumar,et al.  Effect of molecular weight on fibrous PVA produced by electrospinning , 2004 .

[14]  G. Zhizhin,et al.  Handbook of Fourier Transform Raman and Infrared Spectra of Polymers , 1998 .

[15]  D. Briggs,et al.  High resolution XPS of organic polymers , 1992 .

[16]  Lixin Yang,et al.  Biodegradable electrospun fibers for drug delivery. , 2003, Journal of controlled release : official journal of the Controlled Release Society.

[17]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[18]  Guoping Chen,et al.  The use of a novel PLGA fiber/collagen composite web as a scaffold for engineering of articular cartilage tissue with adjustable thickness. , 2003, Journal of biomedical materials research. Part A.

[19]  C. Nah,et al.  Characteristics of polyimide ultrafine fibers prepared through electrospinning , 2003 .