With great interest, we have read the letter ‘Cardiac slowing and acute tryptophan depletion: a comment on the paper by van der Veen et al.’ by Hood et al. This letter is a comment on a recent paper by our group (van der Veen et al. 2008) describing the differential effects of acute tryptophan depletion (ATD) on cardiac and electro-cortical measures of performance monitoring. In the letter, the authors describe two specific cases in which ATD led to serious but transient cardiovascular side effects. These side effects involved significant episodes of bradycardia and in both cases, extra medical help had to be summoned. The authors present the cases as further evidence for an important role of serotonin in the cardiovascular system and underline the importance of cardiovascular screening of subjects participating in ATD procedures. We completely agree with the conclusions and think it is important to take note of the two described cases. However, we would like to stress that the important role of serotonin in cardiovascular regulation is well known (e.g., Jordan 2005; Dergacheva et al. 2007; Ramage and Villalon 2008) and was already briefly mentioned in our paper. Furthermore, the participants in our studies are always thoroughly screened for cardiovascular and other health problems. Finally, the described cases seem to be very atypical and that the cardiovascular side effects are most likely unrelated to the effects on heart rate in our study. Both the described cases had cardiovascular abnormalities before the start of the experiment and perhaps more thorough screening might have led to exclusion of these two patients. Moreover, both cases were psychiatric patients with a history of medication use, which might have contributed to a pre-existing cardiovascular vulnerability. Besides the ATD manipulation, case 2 was also given an infusion of the benzodiazepine antagonist flumazenil, which might have further added to any pre-existing vulnerabilities. Therefore, we think that the described patients are not the typical volunteers who participate in ATD studies, and that a combination of factors might have added to the effects of ATD. In all the studies performed by our group, in which we have included thoroughly screened healthy volunteers with or without a family history of depression, we have never encountered cardiovascular side effects of our procedures. However, it should be noted that we have incidentally encountered physical side effects such as nausea and vomiting and that these side effects have led to incidental dropout. The described cardiovascular side effects are most likely unrelated to the effects described in our study. There is strong evidence for a role of serotonin in the brain circuits involved in feedback processing (e.g., Cools et al. 2008; Jocham and Ullsperger 2009) and cardiac responses to feedback are highly sensitive to subtle changes in activation in these circuits (e.g., Crone et al. 2003; van der Veen et al. 2004). Therefore, we like to argue that the cardiac effects described in our paper are related to changes in these Psychopharmacology (2009) 203:835–836 DOI 10.1007/s00213-008-1429-8
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