Modelling and Implementation of a Novel SPR Biointerface for Time-Effective Detection of Sepsis Biomarkers

Early and effective diagnosis of sepsis is made possible with the detection of specific biomarkers. This requires the design of a specific and sensitive immunoassay biointerface. In this proceeding, we discussed the modelling and implementation of a novel surface plasmon resonance (SPR) interface featuring nano-gratings and selective surface biochemical functionalization. Simulation suggests that such an interface enhances the sensitivity of SPR systems.

[1]  H. Raether.  Surface Plasmons on Smooth and Rough Surfaces and on Gratings , 1988 .

[2]  Helmut Thissen,et al.  Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry. , 2002, Biomaterials.

[3]  G. Clermont,et al.  Epidemiology of severe sepsis in the United States: Analysis of incidence, outcome, and associated costs of care , 2001, Critical care medicine.

[4]  Björn Persson,et al.  Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy. , 2004, Journal of the American Chemical Society.

[5]  D. Heitmann,et al.  Grating couplers for surface plasmons excited on thin metal films in the Kretschmann-Raether configuration , 1999 .

[6]  Bharat Bhushan,et al.  Use of phase imaging in atomic force microscopy for measurement of viscoelastic contrast in polymer nanocomposites and molecularly thick lubricant films. , 2003, Ultramicroscopy.

[7]  G. Whitesides,et al.  Patterning proteins and cells using soft lithography. , 1999, Biomaterials.

[8]  Thomas K. Gaylord,et al.  Rigorous coupled-wave analysis of metallic surface-relief gratings , 1986 .

[9]  D. Mannino,et al.  The epidemiology of sepsis in the United States from 1979 through 2000. , 2003, The New England journal of medicine.

[10]  David T. Crouse,et al.  Nonsteady-state surface plasmons in periodically patterned structures , 2004 .

[11]  Günter Gauglitz,et al.  Surface plasmon resonance sensors: review , 1999 .

[12]  Matthew A. Cooper,et al.  Optical biosensors in drug discovery , 2002, Nature Reviews Drug Discovery.

[13]  A. Conde.  Staphylococcus aureus infections. , 1998, The New England journal of medicine.

[14]  E. Hall,et al.  Surface plasmon resonance: theoretical evolutionary design optimization for a model analyte sensitive absorbing-layer system. , 2004, Analytical chemistry.

[15]  Denise Vera Pollard-Knight,et al.  Modelling of particle-enhanced sensitivity of the surface-plasmon-resonance biosensor , 1994 .

[16]  George Scott,et al.  Toward resolving the challenges of sepsis diagnosis. , 2004, Clinical chemistry.

[17]  A. Kolomenskiǐ,et al.  Sensitivity and detection limit of concentration and adsorption measurements by laser-induced surface-plasmon resonance. , 1997, Applied optics.

[18]  Masahiro Kudo,et al.  Effective monitoring of protein reaction on glass plate surfaces by TOF-SIMS. , 2005, Biosensors & bioelectronics.

[19]  Heather Sheardown,et al.  Polyethylene oxide surfaces of variable chain density by chemisorption of PEO-thiol on gold: adsorption of proteins from plasma studied by radiolabelling and immunoblotting. , 2005, Biomaterials.

[20]  R. Nelson,et al.  Advances in surface plasmon resonance biomolecular interaction analysis mass spectrometry (BIA/MS) , 1999, Journal of molecular recognition : JMR.

[21]  Wolfgang Göpel,et al.  A new affinity biosensor: self-assembled thiols as selective monolayer coatings of quartz crystal microbalances , 1996 .

[22]  R. Rich,et al.  Survey of the year 2004 commercial optical biosensor literature , 2005, Journal of molecular recognition : JMR.

[23]  W. Barnes,et al.  Surface plasmon subwavelength optics , 2003, Nature.

[24]  A. Haes,et al.  A unified view of propagating and localized surface plasmon resonance biosensors , 2004, Analytical and bioanalytical chemistry.