Amino Acid Sequence and Antimicrobial Activity of Chitin-Binding Peptides, Pp-AMP 1 and Pp-AMP 2, from Japanese Bamboo Shoots (Phyllostachys pubescens)

Two novel chitin-binding peptides, designated Pp-AMP 1 and Pp-AMP 2, which had antimicrobial activity against pathogenic bacteria and fungi, were purified from Japanese bamboo shoots (Phyllostachys pubescens) by a simple procedure based on chitin affinity chromatography. They had the common structural features of the plant defensin family, but they could not be grouped in any type of that family. They showed a high degree of homology to mistletoe toxins.

[1]  M. Fujimura,et al.  Purification, Characterization, and Sequencing of Novel Antimicrobial Peptides, Tu-AMP 1 and Tu-AMP 2, from Bulbs of Tulip (Tulipa gesneriana L.) , 2004, Bioscience, biotechnology, and biochemistry.

[2]  M. Fujimura,et al.  Purification, Characterization, and Sequencing of a Novel Type of Antimicrobial Peptides, Fa-AMP1 and Fa-AMP2, from Seeds of Buckwheat (Fagopyrum esculentum Moench.) , 2003, Bioscience, biotechnology, and biochemistry.

[3]  Y. Xiang,et al.  Two novel antifungal peptides distinct with a five‐disulfide motif from the bark of Eucommia ulmoides Oliv , 2002, FEBS letters.

[4]  Jingchu Luo,et al.  Purification, characterization, and molecular cloning of the gene of a seed-specific antimicrobial protein from pokeweed. , 2000, Plant physiology.

[5]  M. Ohnishi-Kameyama,et al.  Binding of Barley and Wheat α-Thionins to Polysaccharides , 2000 .

[6]  Franky R. G. Terras,et al.  Plant Defensins: Novel Antimicrobial Peptides as Components of the Host Defense System , 1995, Plant physiology.

[7]  D. Marshak,et al.  Purification and characterization of a novel antimicrobial peptide from maize (Zea mays L.) kernels. , 1992, The Journal of biological chemistry.

[8]  Franky R. G. Terras,et al.  Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins. , 1992, Biochemistry.

[9]  P. Proost,et al.  Isolation and characterization of a novel class of plant antimicrobial peptides form Mirabilis jalapa L. seeds. , 1992, The Journal of biological chemistry.

[10]  E. Méndez,et al.  γ‐Purothionins: amino acid sequence of two polypeptides of a new family of thionins from wheat endosperm , 1990, FEBS letters.

[11]  H. Schägger,et al.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. , 1987, Analytical biochemistry.

[12]  H. Matsubara,et al.  Amino acid sequence of a purothionin homolog from barley flour. , 1980, Journal of biochemistry.

[13]  G. Samuelsson,et al.  Phoratoxin, a toxic protein from the mistletoe Phoradendron tomentosum subsp. macrophyllum (Loranthaceae). The amino acid sequence. , 1974, Acta pharmaceutica Suecica.

[14]  G. Samuelsson,et al.  Isolation and properties of ligatoxin A, a toxic protein from the mistletoe Phoradendron liga. , 1982, Acta pharmaceutica Suecica.

[15]  G. Samuelsson,et al.  Toxic proteins from the mistletoe Dendrophtora clavata. II. The amino acid sequence of denclatoxin B. , 1977, Acta pharmaceutica Suecica.