论文信息 - Role of Osteogenically Differentiated and Undifferentiated Stem Cells from Adipose Tissue and Bone Marrow on Bone Regeneration in Critical Sized Calvarial Defects

Role of Osteogenically Differentiated and Undifferentiated Stem Cells from Adipose Tissue and Bone Marrow on Bone Regeneration in Critical Sized Calvarial Defects

METHODS: Stem cells were provided from bone marrow and adipose tissue of the rats and osteogenically differentiated.1 Fluorescent labelled (CM-DiI) cells2 were seeded onto gelatin carrier.3 48 rats were equally divided into 6 groups (n=8) and critical sized (8 mm) cranial defect model was used.4 Group 1 had only cranial defect while group 2 recieved gelatin carrier without cells seeded onto. Groups 3 and 4 recieved osteoinduced and non-induced stem cells from adipose tissue and group 5 and 6 recieved osteoinduced and non-induced stem cells derived from bone marrow. Cranial defects were measured with computerized tomography (CT) at 0 (Figure 1), 2nd, 4th and 8th weeks (Figure 2) postoperatively. Rats were sacrifi ced and specimens underwent histological analysis. Stem cells with CM-DiI were visualised. Immunohistochemical labelling with osteocalcin and vWF were performed.

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