Distribution of Ticks and the Risk of Lyme Disease and Other Tick-Borne Pathogens of Public Health Significance in Ontario, Canada.

Over the past two decades, the northward spread of Ixodes scapularis across Ontario, Canada, has accelerated and the risk of Lyme disease has increased. Active surveillance is a recognized and effective method for detecting reproducing populations of I. scapularis. In this study, we conducted field sampling consistent with an active surveillance approach from May to October 2014 at 104 sites in central, eastern, and southern Ontario to determine the current distribution of I. scapularis and other tick species, and enhance our understanding of the geographic risk associated with Borrelia burgdorferi and other tick-borne pathogens of public health significance in this region. I. scapularis was present at 20 of the 104 sites visited. Individuals of the tick species Dermacentor variabilis, Haemaphysalis leporispalustris, and Ixodes dentatus were also collected. I. scapularis was positive by PCR for B. burgdorferi at five sites. These sites formed a significant spatial cluster in eastern Ontario. No ticks were PCR positive for Borrelia miyamotoi, Anaplasma phagocytophilum, and Babesia microti. This study provides an up-to-date picture of the distribution of I. scapularis and other tick species, and the risk of B. burgdorferi and other pathogens of public health significance in central, eastern, and southern Ontario. This information may allow for more effective surveillance efforts and public health interventions for Lyme disease and other tick-borne diseases in this region.

[1]  D. Raoult,et al.  Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[2]  A. Nijhof,et al.  Research on the ecology of ticks and tick-borne pathogens—methodological principles and caveats , 2013, Front. Cell. Infect. Microbiol..

[3]  Bakken Js,et al.  Ehrlichial Diseases of Humans: Emerging Tick-Borne Infections , 1995 .

[4]  L. R. Lindsay,et al.  Methods to Prevent Tick Bites and Lyme Disease. , 2015, Clinics in laboratory medicine.

[5]  M. Bigras-Poulin,et al.  Role of Migratory Birds in Introduction and Range Expansion of Ixodes scapularis Ticks and of Borrelia burgdorferi and Anaplasma phagocytophilum in Canada , 2008, Applied and Environmental Microbiology.

[6]  Nicholas H. Ogden,et al.  Estimated Effects of Projected Climate Change on the Basic Reproductive Number of the Lyme Disease Vector Ixodes scapularis , 2014, Environmental health perspectives.

[7]  N. Ogden,et al.  The prevalence of Borrelia miyamotoi infection, and co-infections with other Borrelia spp. in Ixodes scapularis ticks collected in Canada , 2014, Parasites & Vectors.

[8]  R. Massung,et al.  Multiplex Real-Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi , 2004, Journal of Clinical Microbiology.

[9]  T. Scholten.  Human tick infestations in Ontario: findings at the Toronto Public Health Laboratory, 1967-1977. , 1977, Canadian journal of public health = Revue canadienne de sante publique.

[10]  A. Spielman,et al.  Vectorial capacity of North American Ixodes ticks. , 1984, The Yale journal of biology and medicine.

[11]  Carol A. Gotway,et al.  Visualizing Spatial Data , 2004 .

[12]  J. P. Davis,et al.  Lyme disease: A tick-borne spirochetosis , 1983 .

[13]  L. Trudel,et al.  Passive Surveillance for I. scapularis Ticks: Enhanced Analysis for Early Detection of Emerging Lyme Disease Risk , 2012, Journal of medical entomology.

[14]  A. Nishida,et al.  Babesia microti-group parasites compared phylogenetically by complete sequencing of the CCTeta gene in 36 isolates. , 2009, The Journal of veterinary medical science.

[15]  J S Brownstein,et al.  Spatiotemporal Patterns of Host-Seeking Ixodes scapularis Nymphs (Acari: Ixodidae) in the United States , 2006, Journal of medical entomology.

[16]  H. Artsob,et al.  Spotted Fever Group Rickettsiae: A Brief Review and a Canadian Perspective , 2012, Zoonoses and public health.

[17]  D. Fish,et al.  A relapsing fever group spirochete transmitted by Ixodes scapularis ticks. , 2001, Vector borne and zoonotic diseases.

[18]  Jeff Bowman,et al.  Geography, Deer, and Host Biodiversity Shape the Pattern of Lyme Disease Emergence in the Thousand Islands Archipelago of Ontario, Canada , 2014, PloS one.

[19]  M. Bigras-Poulin,et al.  Risk maps for range expansion of the Lyme disease vector, Ixodes scapularis, in Canada now and with climate change , 2008, International journal of health geographics.

[20]  R. Anderson,et al.  IXODES SCAPULARIS SAY ON WHITE-TAILED DEER (Odocoileus virginianus) FROM LONG POINT, ONTARIO , 1976, Journal of wildlife diseases.

[21]  Badal Dhar,et al.  Population-Based Passive Tick Surveillance and Detection of Expanding Foci of Blacklegged Ticks Ixodes scapularis and the Lyme Disease Agent Borrelia burgdorferi in Ontario, Canada , 2014, PloS one.

[22]  G. Beauchamp,et al.  Investigation of Ground Level and Remote-Sensed Data for Habitat Classification and Prediction of Survival of Ixodes scapularis in Habitats of Southeastern Canada , 2006, Journal of medical entomology.

[23]  A. Hinckley,et al.  Lyme disease testing by large commercial laboratories in the United States. , 2014, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[24]  J. de la Fuente,et al.  The ecology of ticks and epidemiology of tick-borne viral diseases. , 2014, Antiviral research.

[25]  R. Ostfeld,et al.  The function of biodiversity in the ecology of vector-borne zoonotic diseases , 2000 .

[26]  Nicholas H. Ogden,et al.  Active and Passive Surveillance and Phylogenetic Analysis of Borrelia burgdorferi Elucidate the Process of Lyme Disease Risk Emergence in Canada , 2010, Environmental health perspectives.

[27]  M Bigras-Poulin,et al.  A dynamic population model to investigate effects of climate on geographic range and seasonality of the tick Ixodes scapularis. , 2005, International journal for parasitology.

[28]  J. Oliver,et al.  Host Associations and Seasonal Occurrence of Haemaphysalis leporispalustris, Ixodes brunneus, I. cookei, I. dentatus, and I. texanus (Acari: Ixodidae) in Southeastern Missouri , 2003, Journal of medical entomology.

[29]  T. Gillespie,et al.  Survival and development of the different life stages of Ixodes scapularis (Acari: Ixodidae) held within four habitats on Long Point, Ontario, Canada. , 1998, Journal of medical entomology.

[30]  G. Beauchamp,et al.  Associations between Ixodes scapularis ticks and small mammal hosts in a newly endemic zone in southeastern Canada: implications for Borrelia burgdorferi transmission. , 2011, Ticks and tick-borne diseases.

[31]  J. Brownstein,et al.  A climate-based model predicts the spatial distribution of the Lyme disease vector Ixodes scapularis in the United States. , 2003, Environmental health perspectives.

[32]  Y. Pelcat,et al.  Different Ecological Niches for Ticks of Public Health Significance in Canada , 2015, PloS one.

[33]  G. Beauchamp,et al.  Ixodes scapularis Ticks Collected by Passive Surveillance in Canada: Analysis of Geographic Distribution and Infection with Lyme Borreliosis Agent Borrelia burgdorferi , 2006, Journal of medical entomology.