The complete chloroplast genome of Roscoea tibetica, an alpine ginger species

Abstract Roscoea tibetica is a most widespread and extensively phenotypic-variable alpine ginger species. Here, we reported the complete chloroplast (cp) genome sequence of R. tibetica. The complete chloroplast genome is 163,515 base pairs (bp) in length, containing two inverted repeat (IRa and IRb) regions of 29,781 bp, which was separated by a large single-copy (LSC) region of 88,090 bp and a small single-copy (SSC) region of 15,863 bp. The overall GC content of the plastid genome was 36.06%. In total, 113 unique genes were annotated, including 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Phylogenetic analysis based on 22 chloroplast genomes indicates that R. tibetiaca is closely related to R. humeana, forming a monophyletic group with high value.

[1]  Qing‐Jun Li,et al.  An ecological barrier between the Himalayas and the Hengduan Mountains maintains the disjunct distribution of Roscoea , 2019, Journal of Biogeography.

[2]  Jian‐Li Zhao,et al.  The complete chloroplast sequence of Roscoea humeana (Zingiberaceae): an alpine ginger in the Hengduan Mountains, China , 2019, Mitochondrial DNA Part B: Resources.

[3]  M. Burd,et al.  Reproductive isolation in alpine gingers: How do coexisting Roscoea (R. purpurea and R. tumjensis) conserve species integrity? , 2018, Evolution; international journal of organic evolution.

[4]  W. Kress,et al.  Evolutionary diversification of alpine ginger reflects the early uplift of the Himalayan-Tibetan Plateau and rapid extrusion of Indochina , 2016 .

[5]  Alexandros Stamatakis,et al.  RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies , 2014, Bioinform..

[6]  W. Kress,et al.  Reproductive biology of two Himalayan alpine gingers (Roscoea spp., Zingiberaceae) in China: pollination syndrome and compensatory floral mechanisms. , 2011, Plant biology.

[7]  Qing‐Jun Li,et al.  Autonomous selfing provides reproductive assurance in an alpine ginger Roscoea schneideriana (Zingiberaceae). , 2008, Annals of botany.

[8]  Dz Z. Li,et al.  Reassessing the relationships between Gordoniaand Polyspora (Theaceae) based on the combined analyses of molecular data from the nuclear, plastid and mitochondrial genomes , 2004, Plant Systematics and Evolution.

[9]  W. Kress,et al.  The phylogeny and a new classification of the gingers (Zingiberaceae): evidence from molecular data. , 2002, American journal of botany.

[10]  H. Mewes,et al.  BIOINFORMATICS APPLICATIONS NOTE , 2004 .

[11]  E. Pahlich,et al.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue , 1980 .