A landmark-free morphometric staging system for the mouse limb bud

We have created a 2D morphometric analysis of the developing mouse hindlimb bud. This analysis has provided two useful resources for the study of limb development. First, a temporally accurate numerical description of shape changes during normal mouse limb development. Second, a web-based morphometric staging system, which has the advantage of being easy to use, and with a reproducibility of about ±2 hours. It allows users to upload a dorsal-view photo of a limb bud, draw a spline curve and thereby stage the bud within a couple of minutes. We describe how the system is constructed, its robustness to user variation and illustrate one application: the accurate tracking of spatiotemporal dynamics of gene expression patterns.

[1]  D. B. Meyer,et al.  The timing and sequence of events in the development of the human vertebral column during the embryonic period proper , 1979, Anatomy and Embryology.

[2]  S. Bryant,et al.  A staging system for mouse limb development. , 1989, The Journal of experimental zoology.

[3]  E. Boyden,et al.  The timing and sequence of events in the development of the human respiratory system during the embryonic period proper , 2004, Zeitschrift für Anatomie und Entwicklungsgeschichte.

[4]  V. Hamburger,et al.  A series of normal stages in the development of the chick embryo. 1951. , 2012, Developmental dynamics : an official publication of the American Association of Anatomists.

[5]  William H. Press,et al.  Numerical recipes , 1990 .

[6]  F. Bookstein,et al.  The Measurement of Biological Shape and Shape Change. , 1980 .

[7]  Matthew H. Kaufman,et al.  The Atlas of Mouse Development , 1992 .

[8]  J. Hecksher-Sørensen,et al.  Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies , 2002, Science.

[9]  R. Zeller,et al.  Gremlin-mediated BMP antagonism induces the epithelial-mesenchymal feedback signaling controlling metanephric kidney and limb organogenesis , 2004, Development.

[10]  Thomas de Quincey.  [C] , 2000, The Works of Thomas De Quincey, Vol. 1: Writings, 1799–1820.

[11]  R O'Rahilly,et al.  The timing and sequence of events in human cardiogenesis. , 1971, Acta anatomica.

[12]  R Baldock,et al.  A real mouse for your computer. , 1992, BioEssays : news and reviews in molecular, cellular and developmental biology.

[13]  William H. Press,et al.  Numerical recipes in C (2nd ed.): the art of scientific computing , 1992 .

[14]  James Sharpe,et al.  A Computational Clonal Analysis of the Developing Mouse Limb Bud , 2011, PLoS Comput. Biol..

[15]  K. Theiler.  The house mouse. Development and normal stages from fertilization to 4 weeks of age. , 1972 .

[16]  V. Hartenstein.  Atlas of Drosophila development , 1993 .

[17]  Kurt E. Johnson,et al.  Normal Table of Xenopus Laevis , 1968, The Yale Journal of Biology and Medicine.

[18]  H. David Sheets,et al.  Geometric morphometrics for biologists : a primer , 2004 .

[19]  Viktor Hamburger,et al.  A series of normal stages in the development of the chick embryo , 1992, Journal of morphology.

[20]  James Sharpe,et al.  The Role of Spatially Controlled Cell Proliferation in Limb Bud Morphogenesis , 2010, PLoS biology.

[21]  R. O’rahilly,et al.  The timing and sequence of events in the development of the human eye and ear during the embryonic period proper , 2004, Anatomy and Embryology.

[22]  David H. Hall,et al.  C. elegans Atlas , 2008 .

[23]  Ralph Roskies,et al.  Fourier Descriptors for Plane Closed Curves , 1972, IEEE Transactions on Computers.