Extraction with edta/methanol and supercritical carbon dioxide for the analysis of ziram residues on spinach

Two different analysis procedures for Ziram residues on spinach are described. One consists in the extraction of 1 g of lyophilized sample with a 1:1 (v/v) aqueous EDTA‐methanol solution and later partition with hexane as clean‐up. The other procedure involves supercritical carbon dioxide, with the addition of methanol as an organic modifier, to perform the extraction of 0.25 g of lyophilized sample. In both cases, the determination is performed by High Performance Liquid Chromatography and ultraviolet detection. A discussion of both procedures is presented, applying them to the study of the dissipation of Ziram on spinach cultivated in greenhouses at two different environmental temperatures.

[1]  J. Lehotay,et al.  HPLC study of mancozeb degradation on leaves , 1993 .

[2]  J. Bernal,et al.  Use of a high-pressure Soxhlet extractor for the determination of organochlorine residues by gas chromatography , 1992 .

[3]  L. T. Taylor,et al.  Supercritical fluid extraction of active components in a drug formulation. , 1992, Analytical chemistry.

[4]  J. Carson.  Us food and drug administration pesticide program and associated analytical methodology , 1992 .

[5]  M. Jongen,et al.  Gas-liquid and liquid chromatographic determination of zineb and maneb for the assessment of occupational exposure in the production of ornamentals , 1991 .

[6]  G. J. Jong,et al.  Determination of dithiocarbamates in residues by liquid chromatography with selective precolumn or reaction-detection systems. , 1990 .

[7]  K. Markides,et al.  Analytical supercritical fluid chromatography and extraction , 1990 .

[8]  Satya Narayan Naik,et al.  Extraction of perfumes and flavours from plant materials with liquid carbon dioxide under liquid—vapor equilibrium conditions , 1989 .

[9]  Bonnie A. Charpentier,et al.  Supercritical fluid extraction and chromatography : techniques and applications , 1988 .

[10]  M. McNally,et al.  Supercritical fluid extraction coupled with supercritical fluid chromatography for the separation of sulfonylurea herbicides and their metabolites from complex matrices. , 1988, Journal of chromatography.

[11]  R. Boni,et al.  Gas chromatographic determination of dithiocarbamate fungicides in workroom air , 1986, Bulletin of environmental contamination and toxicology.

[12]  J. Lehotay,et al.  High-performance liquid chromatographic determination of dimethyldithiocarbamate residues in some agricultural products. , 1986, Journal of chromatography.

[13]  Reena,et al.  TLC Separation of Some Carbamates on Metal Salt Impregnated Layers , 1983 .

[14]  R. Thompson,et al.  High-pressure liquid chromatographic determination of fungicidal dithiocarbamates. , 1981, Journal of agricultural and food chemistry.

[15]  W. Jennings.  Vapor-phase sampling , 1979 .

[16]  W. Newsome.  A method for determining ethylenebis(dithiocarbamate) residues on food crops as bis(trifluoroacetamido)ethane. , 1974, Journal of agricultural and food chemistry.