Development of an optical sensor based on surface plasmon resonance phenomenon for diagnosis of dengue virus E-protein

Abstract Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface.

[1]  Alain Dufresne,et al.  Nanocellulose in biomedicine: Current status and future prospect , 2014 .

[2]  J. H. Strauss,et al.  Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. , 1985, Science.

[3]  A. Mistchenko,et al.  Complete genome sequencing of dengue virus type 1 isolated in Buenos Aires, Argentina. , 2004, Virus research.

[4]  N. Yusof,et al.  Optical properties of cross-linked chitosan thin film for copper ion detection using surface plasmon resonance technique , 2011 .

[5]  Xuejun Cui,et al.  Synthesis of raspberry-like monodisperse magnetic hollow hybrid nanospheres by coating polystyrene template with Fe(3)O(4)@SiO(2) particles. , 2011, Journal of colloid and interface science.

[6]  C. Sangma,et al.  A novel method for dengue virus detection and antibody screening using a graphene-polymer based electrochemical biosensor. , 2017, Nanomedicine : nanotechnology, biology, and medicine.

[7]  E. Cranston,et al.  Surface modification of cellulose nanocrystals with cetyltrimethylammonium bromide , 2014 .

[8]  Erfan Zalnezhad,et al.  Rapid Immunoglobulin M-Based Dengue Diagnostic Test Using Surface Plasmon Resonance Biosensor , 2014, Scientific Reports.

[9]  Aibing Yu,et al.  Inorganic nanoparticles as carriers for efficient cellular delivery , 2006 .

[10]  Jeho Park,et al.  Surface Plasmon Resonance: A Versatile Technique for Biosensor Applications , 2015, Sensors.

[11]  Di Zhang,et al.  Sonochemical fabrication of Fe3O4 nanoparticles on reduced graphene oxide for biosensors. , 2013, Ultrasonics sonochemistry.

[12]  L. Balan,et al.  A facile method for the preparation of bifunctional Mn:ZnS/ZnS/Fe3O4 magnetic and fluorescent nanocrystals , 2015, Beilstein journal of nanotechnology.

[13]  Christian Bergemann,et al.  Physiological aspects in magnetic drug-targeting , 1999 .

[14]  M. Guzmán,et al.  Dengue diagnosis, advances and challenges. , 2004, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[15]  S. Balakumar,et al.  Influence of physicochemical interactions of capping agent on magnetic properties of magnetite nanoparticles , 2016 .

[16]  M. Rossmann,et al.  Cryo-EM Reconstruction of Dengue Virus in Complex with the Carbohydrate Recognition Domain of DC-SIGN , 2006, Cell.

[17]  Pooja Chawla,et al.  Clinical implications and treatment of dengue. , 2014, Asian Pacific journal of tropical medicine.

[18]  D. Fremont,et al.  Structural insights into the mechanisms of antibody-mediated neutralization of flavivirus infection: implications for vaccine development. , 2008, Cell host & microbe.

[19]  Isdin Oke.  Nanoscience in nature: cellulose nanocrystals , 2010 .

[20]  D.R. Shankaran,et al.  A novel surface plasmon resonance immunosensor for 2,4,6-trinitrotoluene (TNT) based on indirect competitive immunoreaction: a promising approach for on-site landmine detection , 2005, IEEE Sensors Journal.

[21]  Ahmad Ashrif A. Bakar,et al.  Binding Affinity of a Highly Sensitive Au/Ag/Au/Chitosan-Graphene Oxide Sensor Based on Direct Detection of Pb2+ and Hg2+ Ions , 2017, Sensors.

[22]  Zainal Abidin Talib,et al.  Analysis of Pb(II) ion sensing by crosslinked chitosan thin film using surface plasmon resonance spectroscopy , 2013 .