Chlorination contributes to multi-antibiotic resistance in a pilot-scale water distribution system

The generation and dissemination of antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment has become a critical risk to human health. This study is based on a pilot-scale simulated water distribution system to understand the effects of chlorine disinfection treatment (without free chlorine) on ARB and ARGs in biofilms. The hydraulic parameters and pipe materials of the system were simulated based on a drinking water system. The results of the colony counts showed that bacterial multi-antibiotic resistance could be enhanced 13-fold in the biofilms of the pipeline. The use of high-throughput qPCR (HT-qPCR) indicated that the total relative abundance of ARGs in biofilm samples increased significantly (p < 0.05), while the diversity of bacteria was shown to be reduced via taxonomic analysis of the V3–V4 region of 16S rRNA. The prominent types of ARGs were conferred resistance by aminoglycoside and β-lactam after the chlorine disinfection treatment, and antibiotic deactivation was the main mechanism. Phyla Proteobacteria had the highest abundance in both treatment and control groups but decreased from 70.81% (initial biofilm sample) to 26.09% (the sixth-month biofilm sample) in the treatment groups. The results show that the chlorine disinfection plays a role in the risk of development of bacterial antibiotic resistance in pipe networks owing to bacteria in biofilms. This study was the first to investigate the contribution of chlorination without free chlorine to the bacterial community shift and resistome alteration in biofilms at a pilot-test level.

[1]  M. Salavati‐Niasari,et al.  Simple and eco-friendly synthesis of recoverable zinc cobalt oxide-based ceramic nanostructure as high-performance photocatalyst for enhanced photocatalytic removal of organic contamination under solar light , 2021, Separation and Purification Technology.

[2]  M. Salavati‐Niasari,et al.  Enhanced visible-light-driven photocatalytic performance for degradation of organic contaminants using PbWO4 nanostructure fabricated by a new, simple and green sonochemical approach , 2020, Ultrasonics sonochemistry.

[3]  M. Salavati‐Niasari,et al.  Recyclable magnetic ZnCo2O4-based ceramic nanostructure materials fabricated by simple sonochemical route for effective sunlight-driven photocatalytic degradation of organic pollution , 2020 .

[4]  M. Salavati‐Niasari,et al.  Effect of zirconia on improving NOx reduction efficiency of Nd2Zr2O7 nanostructure fabricated by a new, facile and green sonochemical approach , 2020, Ultrasonics sonochemistry.

[5]  Haleema Saleem,et al.  Developments in the Application of Nanomaterials for Water Treatment and Their Impact on the Environment , 2020, Nanomaterials.

[6]  M. Salavati‐Niasari,et al.  Green synthesis of dysprosium stannate nanoparticles using Ficus carica extract as photocatalyst for the degradation of organic pollutants under visible irradiation , 2020 .

[7]  M. Vallet‐Regí,et al.  Nanomaterials as Promising Alternative in the Infection Treatment , 2019, International journal of molecular sciences.

[8]  K. Wan,et al.  Organic carbon: An overlooked factor that determines the antibiotic resistome in drinking water sand filter biofilm. , 2019, Environment international.

[9]  Shenghua Zhang,et al.  Study of biological up-flow roughing filters designed for drinking water pretreatment in rural areas: using ceramic media as filter material , 2018, Environmental technology.

[10]  V. Sourjik,et al.  Autoinducer 2-Dependent Escherichia coli Biofilm Formation Is Enhanced in a Dual-Species Coculture , 2017, Applied and Environmental Microbiology.

[11]  Shenghua Zhang,et al.  Profiling Total Viable Bacteria in a Hemodialysis Water Treatment System. , 2017, Journal of microbiology and biotechnology.

[12]  D. Graham,et al.  Climatic, Geographic and Operational Determinants of Trihalomethanes (THMs) in Drinking Water Systems , 2016, Scientific Reports.

[13]  M. Salavati‐Niasari,et al.  Nanocrystalline barium stannate: facile morphology-controlled preparation, characterization and investigation of optical and photocatalytic properties , 2016, Journal of Materials Science: Materials in Electronics.

[14]  Shenghua Zhang,et al.  Can chlorination co-select antibiotic-resistance genes? , 2016, Chemosphere.

[15]  Chuanfu Zhang,et al.  Relationship between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Acinetobacter baumannii , 2016, Front. Microbiol..

[16]  April Z Gu,et al.  Water Disinfection Byproducts Induce Antibiotic Resistance-Role of Environmental Pollutants in Resistance Phenomena. , 2016, Environmental science & technology.

[17]  M. Kıvanç,et al.  Microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. , 2016, Journal of wound care.

[18]  Xiao-jian Zhang,et al.  Impact of disinfection on drinking water biofilm bacterial community. , 2015, Journal of environmental sciences.

[19]  Tong Zhang,et al.  Bacterial Community Shift Drives Antibiotic Resistance Promotion during Drinking Water Chlorination. , 2015, Environmental science & technology.

[20]  Chengsong Ye,et al.  Induction of bacterial antibiotic resistance by mutagenic halogenated nitrogenous disinfection byproducts. , 2015, Environmental pollution.

[21]  Benjamin D. Stanford,et al.  Evaluating Evidence for Association of Human Bladder Cancer with Drinking-Water Chlorination Disinfection By-Products , 2015, Journal of toxicology and environmental health. Part B, Critical reviews.

[22]  Tong Zhang,et al.  Diversity and functions of bacterial community in drinking water biofilms revealed by high-throughput sequencing , 2015, Scientific Reports.

[23]  Guangxue Wu,et al.  Denitrification and biofilm growth in a pilot-scale biofilter packed with suspended carriers for biological nitrogen removal from secondary effluent. , 2015, Journal of environmental sciences.

[24]  J. Qu,et al.  Characterization of biofilm and corrosion of cast iron pipes in drinking water distribution system with UV/Cl2 disinfection. , 2014, Water research.

[25]  Min Qiao,et al.  High throughput profiling of antibiotic resistance genes in urban park soils with reclaimed water irrigation. , 2014, Environmental science & technology.

[26]  Shenghua Zhang,et al.  Exposure to mutagenic disinfection byproducts leads to increase of antibiotic resistance in Pseudomonas aeruginosa. , 2014, Environmental science & technology.

[27]  J. Vreeburg,et al.  Pyrosequencing reveals bacterial communities in unchlorinated drinking water distribution system: an integral study of bulk water, suspended solids, loose deposits, and pipe wall biofilm. , 2014, Environmental science & technology.

[28]  Min Yang,et al.  Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system. , 2014, Journal of environmental sciences.

[29]  Amy Pruden,et al.  Effect of disinfectant, water age, and pipe materials on bacterial and eukaryotic community structure in drinking water biofilm. , 2014, Environmental science & technology.

[30]  Timothy A. Johnson,et al.  Diverse and abundant antibiotic resistance genes in Chinese swine farms , 2013, Proceedings of the National Academy of Sciences.

[31]  Min Yang,et al.  Pyrosequencing analysis of eukaryotic and bacterial communities in faucet biofilms. , 2012, The Science of the total environment.

[32]  S. Sørensen,et al.  The interconnection between biofilm formation and horizontal gene transfer. , 2012, FEMS immunology and medical microbiology.

[33]  A. Pühler,et al.  The complete genome sequences of four new IncN plasmids from wastewater treatment plant effluent provide new insights into IncN plasmid diversity and evolution. , 2012, Plasmid.

[34]  Otto X. Cordero,et al.  Ecology drives a global network of gene exchange connecting the human microbiome , 2011, Nature.

[35]  M. Kogevinas,et al.  Water disinfection by-products and bladder cancer: is there a European specificity? A pooled and meta-analysis of European case–control studies , 2011, Occupational and Environmental Medicine.

[36]  Heather K. Allen,et al.  Call of the wild: antibiotic resistance genes in natural environments , 2010, Nature Reviews Microbiology.

[37]  S. Krone,et al.  Accounting for mating pair formation in plasmid population dynamics. , 2010, Journal of theoretical biology.

[38]  L. Pilotto.  Disinfection of drinking water, disinfection by-products and cancer: what about Australia? , 2010, Australian journal of public health.

[39]  Yongli Zhang,et al.  Prevalence of Antibiotic Resistance in Drinking Water Treatment and Distribution Systems , 2009, Applied and Environmental Microbiology.

[40]  C. Manaia,et al.  Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water. , 2009, The Science of the total environment.

[41]  G. Cambray,et al.  The SOS Response Controls Integron Recombination , 2009, Science.

[42]  T. D. de Kievit.  Quorum sensing in Pseudomonas aeruginosa biofilms. , 2009, Environmental microbiology.

[43]  Tong Zhang,et al.  Antibiotic resistance genes in water environment , 2009, Applied Microbiology and Biotechnology.

[44]  Tim Driscoll,et al.  Disinfection by-products in drinking water and colorectal cancer: a meta-analysis. , 2007, International journal of epidemiology.

[45]  Amy Pruden,et al.  Antibiotic resistance genes as emerging contaminants: studies in northern Colorado. , 2006, Environmental science & technology.

[46]  J. Mas,et al.  Role of discontinuous chlorination on microbial production by drinking water biofilms. , 2005, Water research.

[47]  M. Ehlers,et al.  Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. , 2004, International journal of food microbiology.

[48]  Ching-Tsan Huang,et al.  Effects of quorum-sensing deficiency on Pseudomonas aeruginosa biofilm formation and antibiotic resistance. , 2002, The Journal of antimicrobial chemotherapy.

[49]  H. Flemming,et al.  Relevance of microbial extracellular polymeric substances (EPSs)--Part I: Structural and ecological aspects. , 2001, Water science and technology : a journal of the International Association on Water Pollution Research.

[50]  L. Kiene,et al.  Poliovirus-1 inactivation and interaction with biofilm: a pilot-scale study , 1997, Applied and environmental microbiology.

[51]  D. Touati,et al.  Hypochlorous acid stress in Escherichia coli: resistance, DNA damage, and comparison with hydrogen peroxide stress , 1996, Journal of bacteriology.

[52]  K. Kobayashi,et al.  Overexpression of the robA gene increases organic solvent tolerance and multiple antibiotic and heavy metal ion resistance in Escherichia coli , 1995, Applied and environmental microbiology.

[53]  R. Seidler,et al.  Selection of antibiotic-resistant standard plate count bacteria during water treatment , 1982, Applied and environmental microbiology.

[54]  R. Seidler,et al.  Antibiotic-resistant bacteria in drinking water , 1981, Applied and environmental microbiology.

[55]  Tong Zhang,et al.  Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water. , 2013, Water Research.

[56]  K. Konstantinidis,et al.  Unprecedented levels of horizontal gene transfer among spatially co-occurring Shewanella bacteria from the Baltic Sea , 2011, The ISME Journal.

[57]  John W. Beaber,et al.  SOS response promotes horizontal dissemination of antibiotic resistance genes , 2004, Nature.

[58]  T. Lekkas,et al.  The occurrence of disinfection by-products in the drinking water of Athens, Greece , 2003, Environmental science and pollution research international.

[59]  R. Finch,et al.  Limitations of presently available glycopeptides in the treatment of Gram-positive infection. , 2001, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.