PURPOSE
To establish a three-dimensional biological printing technique of hBMSCs.
METHODS
The hBMSCs were regularly isolated and cultured, and adjusted to the density of 1x10(6)/mL single cell suspension. Then these cells were labeled by PI fluorescence marker, and transferred by rapid prototype biological printer. Deposition spots were 300microm apart at X-axis, 500microm at Y-axis, 50microm at Z-axis, and then observed by laser confocal microscope.
RESULTS
This technique could deposit cells with good spatial control. In three-dimensional layer, each "cell ink" drop contained 15-35 hBMSCs post-transfer, and achieved accurate distribution with spots distributed 300microm apart at x-axis, 500microm at y-axis and 50microm at Z-axis.
CONCLUSIONS
This study indicates that hBMSCs can be effectively delivered by a rapid prototype printer with speed and accuracy. Application of three dimensional printing is of great importance in tissue engineering bone.