Biocompatibility of Carbonated Hydroxyapatite Nanoparticles with Different Crystallinities

Carbonated apatite (CHA) is commonly considered a promising synthetic material for biomedical applications in orthopedic and dental surgery due to its biocompatibility, bioresorption and bioactivity. CHA5, CHA37 and CHA90 powders were synthesized from wet method and the DRX patterns showed that the crystallinity and particle size of CHA samples increased proportionally with the synthesis temperature. Powder extracts medium were obtained from each sample to interact with MC3T3-E1 osteoblastics cells. It was evaluated morphology, citotoxicity, pH and Ca2+ concentration. Citotoxicity assays showed high metabolic activity on all samples when compared to control. The polygonal shaped and the confluent monolayer observed in control cells progressively changed according to the crystallinity increase of samples. Cells under mitosis and spindle-like shaped where the main alterations observed. In addition the cell viability could be sensitive to the acid reactivity and crystallinity of carbonated apatite samples.

[1]  Benjamin M. Wu,et al.  Osteoblast Interactions Within a Biomimetic Apatite Microenvironment , 2011, Annals of Biomedical Engineering.

[2]  A. Rossi,et al.  Structural Properties of Nanostructured Carbonate Apatites , 2008 .

[3]  D. Bernache-Assollant,et al.  Processing of AB-type carbonated hydroxyapatite Ca10−x(PO4)6−x(CO3)x(OH)2−x−2y(CO3)y ceramics with controlled composition , 2008 .

[4]  C. Morelli,et al.  A Novel Genetically Engineered Human Osteoblasts For The In Vitro Study Of Biomaterials , 2008 .

[5]  M. Mazzocchi,et al.  Synthesis of carbonated hydroxyapatites: efficiency of the substitution and critical evaluation of analytical methods , 2005 .

[6]  D. Ward,et al.  Physiological changes in extracellular calcium concentration directly control osteoblast function in the absence of calciotropic hormones. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S. Koutsopoulos,et al.  Synthesis and characterization of hydroxyapatite crystals: a review study on the analytical methods. , 2002, Journal of biomedical materials research.

[8]  I. Shapiro,et al.  Matrix Regulation of Skeletal Cell Apoptosis , 2001, The Journal of Biological Chemistry.

[9]  I. Shapiro,et al.  MATRIX REGULATION OF SKELETAL CELL APOPTOSIS: ROLE OF CALCIUM AND PHOSPHATE IONS , 2001 .

[10]  M. H. Fernandes,et al.  In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings , 1999, Journal of materials science. Materials in medicine.

[11]  R Z LeGeros,et al.  Calcium phosphates in oral biology and medicine. , 1991, Monographs in oral science.

[12]  C. P. Shields,et al.  Acid Reactivity of Carbonated Apatites with Strontium and Fluoride Substitutions , 1983, Journal of dental research.