Partial Purification and Characterization of Raucaffricine β‐D‐Glucosidase from Plant Cell‐Suspension Cultures of Rauwolfia serpentina BENTH

A novel highly substrate-specific Rauwolfia enzyme, raucaffricine β-D-glucosidase, was isolated from cell-suspension cultures of R. serpentina. The enzyme has been purified ca. 1350-fold, its major characteristics such as Mr = 66600 ± 5%, pH optimum 5.1, temperature optimum 38°, and inhibition of its activity by glucose and fructose were investigated. Its limited distribution in different cell cultures and differentiated plants indicates that the enzyme is present in significant amounts exclusively in cultured Rauwolfia cells.

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