Organization of the Cytoskeleton in Brine Shrimp Setal Cells is Molt-Dependent

Fluorescence microscopy was used to examine the cytoskeleton in setal cells and antennae of the brine shrimp Artemia franciscana. Each setal cell has an elongated apical process that contains bundles of microtubules and microfilaments. When the organism molts, the apical process telescopes reversibly through the setal cell body into the hemocoel of the antenna. Staining of larval-stage Artemia with four monoclonal anti-tubulin antibodies (DM1 A, TAT, YL1/2, KMX) and with rhodamine–phalloidin indicated that the cytoskeletal elements were stable, remaining assembled as co-localized bundles in telescoping setal cells. Microtubule stability was suggested by previous observations of detyrosinated tubulin in setal cell extensions, but the microtubules were not completely detyrosinated, as shown by their interaction with YL1/2. Foci of tubulin staining within the antenna, enrichment of the microfilaments associated with the invaginating setal cell membrane, and the spatial distribution of other cytoskeletal elem...

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