Proliferation and viability of adherent cells manipulated by standing-wave ultrasound in a microfluidic chip.

Ultrasonic-standing-wave (USW) technology has potential to become a standard method for gentle and contactless cell handling in microfluidic chips. We investigate the viability of adherent cells exposed to USWs by studying the proliferation rate of recultured cells following ultrasonic trapping and aggregation of low cell numbers in a microfluidic chip. The cells form 2-D aggregates inside the chip and the aggregates are held against a continuous flow of cell culture medium perpendicular to the propagation direction of the standing wave. No deviations in the doubling time from expected values (24 to 48 h) were observed for COS-7 cells held in the trap at acoustic pressure amplitudes up to 0.85 MPa and for times ranging between 30 and 75 min. Thus, the results demonstrate the potential of ultrasonic standing waves as a tool for gentle manipulation of low cell numbers in microfluidic systems.

[1]  S. Bhatia,et al.  An extracellular matrix microarray for probing cellular differentiation , 2005, Nature Methods.

[2]  Victor Steinberg,et al.  Continuous particle size separation and size sorting using ultrasound in a microchannel , 2006 .

[3]  Jeremy J Hawkes,et al.  Ultrasonic deposition of cells on a surface. , 2004, Biosensors & bioelectronics.

[4]  Mattias Goksör,et al.  Optical tweezers applied to a microfluidic system. , 2004, Lab on a chip.

[5]  H M Hertz,et al.  Ultrasonic standing wave manipulation technology integrated into a dielectrophoretic chip. , 2006, Lab on a chip.

[6]  Robert W Barber,et al.  Continuous cell washing and mixing driven by an ultrasound standing wave within a microfluidic channel. , 2004, Lab on a chip.

[7]  Holly M. Brown-Borg,et al.  Culture of Animal Cells: A Manual of Basic Technique, R. Ian Freshney (Ed.). Wiley-Liss, Inc., New York (1994) , 1995 .

[8]  W. Coakley,et al.  Enhanced sedimentation of mammalian cells following acoustic aggregation. , 1989, Biotechnology and bioengineering.

[9]  J. Taylor‐Papadimitriou,et al.  Culture of Animal Cells. A manual for basic techniques , 2000 .

[10]  Neil M. White,et al.  A silicon microfluidic ultrasonic separator , 2003 .

[11]  J M Piret,et al.  Batch and Semicontinuous Aggregation and Sedimentation of Hybridoma Cells by Acoustic Resonance Fields , 1995, Biotechnology progress.

[12]  Kenji Yasuda.  Non-destructive, non-contact handling method for biomaterials in micro-chamber by ultrasound , 2000 .

[13]  Larisa A. Kuznetsova,et al.  Microparticle concentration in short path length ultrasonic resonators: Roles of radiation pressure and acoustic streaming , 2004 .

[14]  H M Hertz,et al.  Ultrasonic enhancement of bead-based bioaffinity assays. , 2006, Lab on a chip.

[15]  Thomas Laurell,et al.  Carrier medium exchange through ultrasonic particle switching in microfluidic channels. , 2005, Analytical chemistry.

[16]  D E H Tee,et al.  Book Review: Culture of Animal Cells: A Manual of Basic Technique , 1984 .

[17]  Stefan Radel,et al.  A new immobilisation method to arrange particles in a gel matrix by ultrasound standing waves. , 2005, Ultrasound in medicine & biology.

[18]  E. Benes,et al.  Viability of yeast cells in well controlled propagating and standing ultrasonic plane waves. , 2000, Ultrasonics.

[19]  W. Burger,et al.  A Novel Ultrasonic Resonance Field Device for the Retention of Animal Cells , 1994, Biotechnology progress.

[20]  L. Gor’kov,et al.  On the forces acting on a small particle in an acoustical field in an ideal fluid , 1962 .

[21]  Donald L Feke,et al.  Retention and Viability Characteristics of Mammalian Cells in an Acoustically Driven Polymer Mesh , 2004, Biotechnology progress.

[22]  N. Amso,et al.  Fluorescein isothiocynate-dextran uptake by chinese hamster ovary cells in a 1.5 MHz ultrasonic standing wave in the presence of contrast agent. , 2006, Ultrasound in medicine & biology.

[23]  Despina Bazou,et al.  Physical enviroment of 2-D animal cell aggregates formed in a short pathlength ultrasound standing wave trap. , 2005, Ultrasound in medicine & biology.

[24]  Despina Bazou,et al.  Molecular adhesion development in a neural cell monolayer forming in an ultrasound trap , 2005, Molecular membrane biology.

[25]  Adrian Neild,et al.  Positioning, displacement, and localization of cells using ultrasonic forces. , 2005, Biotechnology and bioengineering.

[26]  P J Keay,et al.  Plasma preparation from whole blood using ultrasound. , 2000, Ultrasound in medicine & biology.

[27]  Amine Kamen,et al.  Acoustic cell filter: a proven cell retention technology for perfusion of animal cell cultures. , 2004, Biotechnology advances.

[28]  Thomas Laurell,et al.  Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels. , 2004, The Analyst.

[29]  R. Gentz,et al.  High-density perfusion culture of insect cells with a biosep ultrasonic filter. , 1998, Biotechnology and bioengineering.

[30]  Gabriele Gradl,et al.  The potential of dielectrophoresis for single-cell experiments. , 2003, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society.