Antibacterial activity of Hibiscus rosa-sinensis L. red flower against antibiotic-resistant strains of Helicobacter pylori and identification of the flower constituents

Utilization of plant resources for treatment of Helicobacter pylori infections is one of the appealing approaches as rapid emergence of antibiotic-resistant strains is occurring throughout the world. Ethanol extract and its fractions from Hibiscus rosa-sinensis red flower were assessed for antibacterial and urease inhibitory activities towards forty-three clinical strains and two reference strains of H. pylori. The ethyl acetate fraction exhibited the most potent bacteriostatic activity with minimum inhibitory concentrations (MICs) of 0.2-0.25 mg/mL and minimum bactericidal concentrations (MBCs) of 1.25-1.5 mg/mL against all test strains, including forty-three strains resistant to one to four antibiotics, azithromycin (MICs, 8-256 µg/mL), erythromycin (MICs, 8-128 µg/mL), levofloxacin (MICs, 8-256 µg/mL), and/or metronidazole (MICs, 8-256 µg/mL). The fraction had similar antibacterial activities toward these test strains suggesting the preparation and the antibiotics do not have a common mechanism of anti-H. pylori activity. The fraction also had stronger effects on biofilm formation, morphological conversion, and urease activity of H. pylori than the other fractions and the ethanol extract. These flower preparations were non-toxic to three human cell lines, and nine compounds were also isolated and identified from the ethyl acetate fraction. In vivo research needs to be conducted to confirm the potential usefulness of H. rosa-sinensis flower and its constituents for effective prevention and treatment of H. pylori disease.

[1]  Hoang Nguyen Van Minh,et al.  Growth-Inhibiting, Bactericidal, Antibiofilm, and Urease Inhibitory Activities of Hibiscus rosa sinensis L. Flower Constituents toward Antibiotic Sensitive- and Resistant-Strains of Helicobacter pylori , 2020, ACS omega.

[2]  A. Di Leo,et al.  The Puzzle of Coccoid Forms of Helicobacter pylori: Beyond Basic Science , 2020, Antibiotics.

[3]  D. Baker.  Plants against Helicobacter pylori to combat resistance: An ethnopharmacological review , 2020, Biotechnology reports.

[4]  A. Di Pizio,et al.  Bitterless guaifenesin prodrugs—design, synthesis, characterization, in vitro kinetics, and bitterness studies , 2018, Chemical biology & drug design.

[5]  R. Alizadeh-Navaei,et al.  Systematic review with meta‐analysis: the worldwide prevalence of Helicobacter pylori infection , 2018, Alimentary pharmacology & therapeutics.

[6]  H. Kiyono,et al.  Characterization of morphological conversion of Helicobacter pylori under anaerobic conditions , 2018, Microbiology and immunology.

[7]  A. Al-Snafi.  Chemical constituents, pharmacological effects and therapeutic importance of Hibiscus rosa-sinensis- A review , 2018 .

[8]  A. Missoum.  Hibiscus rosa sinensis phytochemistry and medicinal uses , 2018 .

[9]  P. Alam,et al.  PHYTOCHEMICAL INVESTIGATIONS OF THE LEAVES AND FLOWERS OF HIBISCUS ROSA-SINENSIS L. , 2017, INDIAN DRUGS.

[10]  L. T. Ngan,et al.  Antibacterial activity of ethanolic extracts of some Vietnamese medicinal plants against Helicobacter pylori , 2017 .

[11]  H. P. Hernandez,et al.  Two Antibacterial Compounds: Velutin and 4- (Hydroxy (Oxiran-2-yl)Methyl)-2-Methoxyphenol from the Stem Bark of Drimys arfakensis Gibbs. , 2017 .

[12]  S. Mukanganyama,et al.  Isolation and characterization of an antifungal compound 5-hydroxy-7,4’-dimethoxyflavone from Combretum zeyheri , 2015, BMC Complementary and Alternative Medicine.

[13]  A. Yadav,et al.  Acute and sub-acute oral toxicity assessment of the methanolic extract from leaves of Hibiscus rosa-sinensis L. in mice , 2014, Journal of intercultural ethnopharmacology.

[14]  Y. Ahn,et al.  Growth Inhibitory, Bactericidal, and Morphostructural Effects of Dehydrocostus Lactone from Magnolia sieboldii Leaves on Antibiotic-Susceptible and -Resistant Strains of Helicobacter pylori , 2014, PloS one.

[15]  Asad U. Khan,et al.  Inhibition of Major Virulence Pathways of Streptococcus mutans by Quercitrin and Deoxynojirimycin: A Synergistic Approach of Infection Control , 2014, PloS one.

[16]  I. Alshami,et al.  Hibiscus sabdariffa extract inhibits in vitro biofilm formation capacity of Candida albicans isolated from recurrent urinary tract infections. , 2014, Asian Pacific journal of tropical biomedicine.

[17]  G. Mandalari,et al.  Antibacterial activities of almond skins on cagA-positive and-negative clinical isolates of Helicobacter pylori , 2013, BMC Microbiology.

[18]  Dung Quoc Ho,et al.  The Incidence of Primary Antibiotic Resistance of Helicobacter pylori in Vietnam , 2013, Journal of clinical gastroenterology.

[19]  T. Mehmood,et al.  Anti-Helicobacter pylori and Urease Inhibition Activities of Some Traditional Medicinal Plants , 2013, Molecules.

[20]  Li‐jun Wu,et al.  Molecular docking, kinetics study, and structure-activity relationship analysis of quercetin and its analogous as Helicobacter pylori urease inhibitors. , 2012, Journal of agricultural and food chemistry.

[21]  T. Shibamoto,et al.  Growth-inhibiting, bactericidal, and urease inhibitory effects of Paeonia lactiflora root constituents and related compounds on antibiotic-susceptible and -resistant strains of Helicobacter pylori. , 2012, Journal of agricultural and food chemistry.

[22]  Chaoyang Ma,et al.  p-Coumaric acid kills bacteria through dual damage mechanisms , 2012 .

[23]  K. Gajalakshmi,et al.  In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens. , 2012, Asian Pacific journal of tropical biomedicine.

[24]  T. Shibamoto,et al.  Growth-inhibiting effects of Paeonia lactiflora root steam distillate constituents and structurally related compounds on human intestinal bacteria , 2012, World journal of microbiology & biotechnology.

[25]  D. Sakarkar,et al.  Pharmacological effects of aqueous-ethanolic extract of Hibiscus rosasinensis on volume and acidity of stimulated gastric secretion. , 2011, Asian Pacific journal of tropical medicine.

[26]  Shadia M. Azzam,et al.  Polyphenolic Compounds from Flowers of Hibiscus rosa-sinensis Linn. and their Inhibitory Effect on Alkaline Phosphatase Enzyme Activity in vitro , 2011, Zeitschrift fur Naturforschung. C, Journal of biosciences.

[27]  B. Kim,et al.  Antibiotic Resistance in Helicobacter pylori Infection , 2011 .

[28]  A. M. Uyub.  Invitro anti-metronidazole-resistant-Helicobacter pylori activity and cytotoxicity of selected medicinal plants’ extracts from Penang Island Malaysia. , 2010 .

[29]  B. Uddin,et al.  Antibacterial activity of the ethanol extracts of Hibiscus rosa-sinensis leaves and flowers against clinical isolates of bacteria , 2010 .

[30]  M. Blaser,et al.  Coadaptation of Helicobacter pylori and humans: ancient history, modern implications. , 2009, The Journal of clinical investigation.

[31]  V. Kadam,et al.  Traditional medicinal uses of Hibiscus rosa-sinensis , 2009 .

[32]  Y. Ajani,et al.  Phenolic compounds from Peucedanum ruthenicum M. Bieb , 2009 .

[33]  A. Ghaemi,et al.  In vitro inhibition of Helicobacter pylori urease with non and semi fermented Camellia sinensis. , 2009, Indian journal of medical microbiology.

[34]  I. Raskin,et al.  Plants and human health in the twenty-first century. , 2002, Trends in biotechnology.

[35]  D. Guiney,et al.  Effect of nitric oxide on Helicobacter pylori morphology. , 1999, The Journal of infectious diseases.

[36]  K. Mabe,et al.  In Vitro and In Vivo Activities of Tea Catechins against Helicobacter pylori , 1999, Antimicrobial Agents and Chemotherapy.

[37]  J. Gisbert,et al.  Mutations in 23S rRNA in Helicobacter pylori Conferring Resistance to Erythromycin Do Not Always Confer Resistance to Clarithromycin , 1999, Antimicrobial Agents and Chemotherapy.

[38]  J. Y. Cho,et al.  Antimicrobial activity of 4-hydroxybenzoic acid and trans 4-hydroxycinnamic acid isolated and identified from rice hull. , 1998, Bioscience, biotechnology, and biochemistry.

[39]  P. Okemo,et al.  Activity of east African medicinal plants against Helicobacter pylori. , 1996, Chemotherapy.