Conversion of methionine to Xanthomonas campestris pv. phytotoxic 3-methylthiopropionic acid by

Incubation of the plant pathogenic bacterium Xanthomonas campestris pv. mmihotis with either ~-[3,414C]methionine or [3,4- 4C]KMBA (2-keto-4-methylthiobutyric acid) led to the production of [l 4C]MTPA (3methylthiopropionic acid) and [ 4C]MTAA (3methylthioacrylic acid). When an excess of non-radioactive KMBA was present in the medium, formation of [ 14C]KMBA from ~-[3,4-~~C]methionhe was observed. Conversely, [ 14C]methionhe accumulated in a trapping pool of non-radioactive methionhe as a result of biotransformation of [3,4-14C]KMBA. Aminooxyacetic acid, a transaminase inhibitor, suppressed totally the formation of [ 4C]MTPA from ~-[3,4-l 4C]methionine but not from [3,4-' 4C]KMBA. Metabolism of L-[ l-14C]methionine liberated 4C02 but did not produce [ l4C]MTPA. These results demonstrate that methionhe is catabolized by X. campestris pv. mmihotis into MTPA via transamination and subsequent decarboxylation of the intermediate a-keto acid KMBA.

[1]  C. Knowles,et al.  Ethylene formation by cell-free extracts of Escherichia coli , 1986, Archives of Microbiology.

[2]  C. Knowles,et al.  Ethylene formation by cultures of Escherichia coli , 1985, Archives of Microbiology.

[3]  H. Maraite,et al.  Detection of 3(methylthio) Propionic-acid in Cassava Leaves Infected By Xanthomonas-campestris Pv Manihotis , 1986 .

[4]  D. Robeson,et al.  Production of low molecular weight carboxylic acids by Xanthomonas campestris pv. campestris in relation to the amino acid composition of the medium and their possible involvement in pathogenesis , 1985 .

[5]  H. Maraite,et al.  Identification of 3(methylthio) propionic acid as a blight-inducing toxin produced by Xanthomonas campestris pv. manihotis in vitro , 1982 .

[6]  S. Iwasaki,et al.  Isolation and Structural Elucidation of Phytotoxic Substances Produced by Xanthomonas campestris pv. oryzae (Ishiyama) Dye , 1980 .

[7]  J. Dixon,et al.  The decarboxylation of α-keto-γ-methiolbutyrate in rat liver mitochondria , 1980 .

[8]  N. Benevenga,et al.  Identification of 3-methylthiopropionic acid as an intermediate in mammalian methionine metabolism in vitro. , 1978, The Journal of biological chemistry.

[9]  C. Panagopoulos,et al.  A proposed nomenclature and classification for plant pathogenic bacteria , 1978 .

[10]  A. Mitchell,et al.  The role of transamination in methionine oxidation in the rat. , 1978, The Journal of nutrition.

[11]  G. L. Case,et al.  Evidence for S-adenosylmethionine independent catabolism of methionine in the rat. , 1976, The Journal of nutrition.

[12]  H. Maraite,et al.  Xanthomonas manihotis (Arthaud-Berthet) Starr, Causal Agent of Bacterial Wilt, Blight and Leaf Spots of Cassava in Zaire , 1975 .

[13]  R. Rognstad,et al.  Effects of aminooxyacetate on the metabolism of isolated liver cells. , 1974, Archives of biochemistry and biophysics.

[14]  K. Arima,et al.  Studies on Substances Active on the Behavior of Planarian:Part I. Production of trans -3-Methylthioacrylic Acid and 3-Methylthiopropionic Acid by Microorganisms , 1970 .

[15]  D. Dye.  The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. , 1962 .

[16]  S. Moore,et al.  Automatic recording apparatus for use in the chromatography of amino acids. , 1958, Federation proceedings.