miRNAs and long noncoding RNAs as biomarkers in human diseases

Noncoding RNAs (ncRNAs) are transcripts that have no apparent protein-coding capacity; however, many ncRNAs have been found to play a major biological role in human physiology. Their deregulation is implicated in many human diseases, but their exact roles are only beginning to be elucidated. Nevertheless, ncRNAs are extensively studied as a novel source of biomarkers, and the fact that they can be detected in body fluids makes them extremely suitable for this purpose. The authors mainly focus on ncRNAs as biomarkers in cancer, but also touch on other human diseases such as cardiovascular diseases, autoimmune diseases, neurological disorders and infectious diseases. The authors discuss the established methods and provide a selection of emerging new techniques that can be used to detect and quantify ncRNAs. Finally, the authors discuss ncRNAs as a new strategy for therapeutic interventions.

[1]  A. Feinberg,et al.  Hypomethylation distinguishes genes of some human cancers from their normal counterparts , 1983, Nature.

[2]  V. Ambros,et al.  The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 , 1993, Cell.

[3]  G. Ruvkun,et al.  Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans , 1993, Cell.

[4]  R. Sun,et al.  Polyadenylylated nuclear RNA encoded by Kaposi sarcoma-associated herpesvirus. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[5]  W. Zhong,et al.  Characterization of ribonucleoprotein complexes containing an abundant polyadenylated nuclear RNA encoded by Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) , 1997, Journal of virology.

[6]  W. Isaacs,et al.  DD3: a new prostate-specific gene, highly overexpressed in prostate cancer. , 1999, Cancer research.

[7]  L. Lim,et al.  An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans , 2001, Science.

[8]  J. Squire,et al.  Identification of a novel gene NCRMS on chromosome 12q21 with differential expression between Rhabdomyosarcoma subtypes , 2002, Oncogene.

[9]  D. Alarcón-Segovia,et al.  A major susceptibility locus for systemic lupus erythemathosus maps to chromosome 1q31. , 2002, American journal of human genetics.

[10]  Hengbin Wang,et al.  Role of Histone H3 Lysine 27 Methylation in X Inactivation , 2003, Science.

[11]  Michael Thomas,et al.  MALAT-1, a novel noncoding RNA, and thymosin β4 predict metastasis and survival in early-stage non-small cell lung cancer , 2003, Oncogene.

[12]  B. Tsao.  The genetics of human systemic lupus erythematosus. , 2003, Trends in immunology.

[13]  C. Croce,et al.  Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[14]  S. Shirasawa,et al.  SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. , 2004, Human molecular genetics.

[15]  B. Tsao.  Update on human systemic lupus erythematosus genetics , 2004, Current opinion in rheumatology.

[16]  Martina Tinzl,et al.  DD3PCA3 RNA analysis in urine--a new perspective for detecting prostate cancer. , 2004, European urology.

[17]  C. Croce,et al.  MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[18]  S. Salzberg,et al.  The Transcriptional Landscape of the Mammalian Genome , 2005, Science.

[19]  Muller Fabbri,et al.  A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. , 2005, The New England journal of medicine.

[20]  D. Reidel,et al.  The Transcriptional Landscape of the Mammalian Genome The FANTOM Consortium* and RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)* , 2005 .

[21]  Wigard P Kloosterman,et al.  In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes , 2005, Nature Methods.

[22]  Sonal Patel,et al.  A single-molecule method for the quantitation of microRNA gene expression , 2005, Nature Methods.

[23]  H. Horvitz,et al.  MicroRNA expression profiles classify human cancers , 2005, Nature.

[24]  N. Rajewsky,et al.  Silencing of microRNAs in vivo with ‘antagomirs’ , 2005, Nature.

[25]  L. Kemény,et al.  Identification and Characterization of a Novel, Psoriasis Susceptibility-related Noncoding RNA gene, PRINS* , 2005, Journal of Biological Chemistry.

[26]  S. Horswell,et al.  Overlapping BXSB congenic intervals, in combination with microarray gene expression, reveal novel lupus candidate genes , 2006, Genes and Immunity.

[27]  Gene W. Yeo,et al.  Noncoding RNAs in the mammalian central nervous system. , 2006, Annual review of neuroscience.

[28]  Shiping Fang,et al.  Attomole microarray detection of microRNAs by nanoparticle-amplified SPR imaging measurements of surface polyadenylation reactions. , 2006, Journal of the American Chemical Society.

[29]  Peter A. Jones,et al.  Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells. , 2006, Cancer cell.

[30]  W. Filipowicz,et al.  Relief of microRNA-Mediated Translational Repression in Human Cells Subjected to Stress , 2006, Cell.

[31]  Zhiqiang Gao,et al.  Detection of microRNAs using electrocatalytic nanoparticle tags. , 2006, Analytical chemistry.

[32]  Meng Li,et al.  Rapid Identification of UCA1 as a Very Sensitive and Specific Unique Marker for Human Bladder Carcinoma , 2006, Clinical Cancer Research.

[33]  Tara L. Naylor,et al.  microRNAs exhibit high frequency genomic alterations in human cancer. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[34]  M. Noguchi,et al.  Phenotypic characterization of endometrial stromal sarcoma of the uterus , 2006, Cancer science.

[35]  C. Croce,et al.  MicroRNA signatures in human cancers , 2006, Nature Reviews Cancer.

[36]  F. Slack,et al.  Oncomirs — microRNAs with a role in cancer , 2006, Nature Reviews Cancer.

[37]  Igor Jurisica,et al.  The c-Myc oncogene directly induces the H19 noncoding RNA by allele-specific binding to potentiate tumorigenesis. , 2006, Cancer research.

[38]  Alan M. Moses,et al.  In vivo enhancer analysis of human conserved non-coding sequences , 2006, Nature.

[39]  Yusuke Nakamura,et al.  Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction , 2006, Journal of Human Genetics.

[40]  P. Sarnow,et al.  Interaction of viruses with the mammalian RNA interference pathway. , 2006, Virology.

[41]  M. Fraga,et al.  Genetic unmasking of an epigenetically silenced microRNA in human cancer cells. , 2007, Cancer research.

[42]  M. Karin,et al.  A large noncoding RNA is a marker for murine hepatocellular carcinomas and a spectrum of human carcinomas , 2007, Oncogene.

[43]  A. De la taille.  Progensa™ PCA3 test for prostate cancer detection , 2007, Expert review of molecular diagnostics.

[44]  H. Sültmann,et al.  The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function. , 2007, Cancer research.

[45]  Zhiqiang Gao,et al.  Direct labeling microRNA with an electrocatalytic moiety and its application in ultrasensitive microRNA assays. , 2007, Biosensors & bioelectronics.

[46]  Reuven Agami,et al.  RNA-Binding Protein Dnd1 Inhibits MicroRNA Access to Target mRNA , 2007, Cell.

[47]  Thomas D. Schmittgen,et al.  Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas. , 2007, Cancer cell.

[48]  L. Lim,et al.  A microRNA component of the p53 tumour suppressor network , 2007, Nature.

[49]  L. Bernd,et al.  Prognostic significance of drug-regulated genes in high-grade osteosarcoma , 2007, Modern Pathology.

[50]  William Stafford Noble,et al.  Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project , 2007, Nature.

[51]  D. Banerjee,et al.  A miR-24 microRNA binding-site polymorphism in dihydrofolate reductase gene leads to methotrexate resistance , 2007, Proceedings of the National Academy of Sciences.

[52]  Hyman M. Schipper,et al.  MicroRNA Expression in Alzheimer Blood Mononuclear Cells , 2007, Gene regulation and systems biology.

[53]  P. Stadler,et al.  RNA Maps Reveal New RNA Classes and a Possible Function for Pervasive Transcription , 2007, Science.

[54]  T. Fojo.  Multiple paths to a drug resistance phenotype: mutations, translocations, deletions and amplification of coding genes or promoter regions, epigenetic changes and microRNAs. , 2007, Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy.

[55]  Leonard D. Goldstein,et al.  MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype , 2007, Genome Biology.

[56]  Xiaoqing Tang,et al.  A simple array platform for microRNA analysis and its application in mouse tissues. , 2007, RNA.

[57]  T. Kwok,et al.  Riboregulator H19 induction of MDR1-associated drug resistance in human hepatocellular carcinoma cells , 2007, Oncogene.

[58]  K. Zatloukal,et al.  Characterization of HULC, a novel gene with striking up-regulation in hepatocellular carcinoma, as noncoding RNA. , 2007, Gastroenterology.

[59]  T. Kwok,et al.  Induction of drug resistance and transformation in human cancer cells by the noncoding RNA CUDR. , 2007, RNA.

[60]  Margaret S. Ebert,et al.  MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells , 2007, Nature Methods.

[61]  S. Eccles,et al.  Metastasis: recent discoveries and novel treatment strategies , 2007, The Lancet.

[62]  Howard Y. Chang,et al.  Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs , 2007, Cell.

[63]  J. Lötvall,et al.  Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells , 2007, Nature Cell Biology.

[64]  Michael D. Schneider,et al.  Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure , 2008, Proceedings of the National Academy of Sciences.

[65]  Tsung-Cheng Chang,et al.  Widespread microRNA repression by Myc contributes to tumorigenesis , 2008, Nature Genetics.

[66]  Suresh Shrestha,et al.  Bioluminescence-based detection of microRNA, miR21 in breast cancer cells. , 2008, Analytical chemistry.

[67]  Francis S Collins,et al.  A HapMap harvest of insights into the genetics of common disease. , 2008, The Journal of clinical investigation.

[68]  Jian Gu,et al.  Single Nucleotide Polymorphisms of microRNA Machinery Genes Modify the Risk of Renal Cell Carcinoma , 2008, Clinical Cancer Research.

[69]  F. Hirsch,et al.  EGFR regulation by microRNA in lung cancer: correlation with clinical response and survival to gefitinib and EGFR expression in cell lines. , 2008, Annals of oncology : official journal of the European Society for Medical Oncology.

[70]  Yuriy Gusev,et al.  Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors. , 2007, RNA.

[71]  C. Carlson,et al.  Multiple Independent Genetic Variants in the 8q24 Region Are Associated with Prostate Cancer Risk , 2008, Cancer Epidemiology Biomarkers & Prevention.

[72]  G. Calin,et al.  Cancer-associated genomic regions (CAGRs) and noncoding RNAs: bioinformatics and therapeutic implications , 2008, Mammalian Genome.

[73]  R. Aharonov,et al.  MicroRNAs accurately identify cancer tissue origin , 2008, Nature Biotechnology.

[74]  A. Roses,et al.  Identification of miRNA Changes in Alzheimer's Disease Brain and CSF Yields Putative Biomarkers and Insights into Disease Pathways , 2008 .

[75]  C. Esau,et al.  Inhibition of microRNA with antisense oligonucleotides. , 2008, Methods.

[76]  Q. Lu,et al.  MicroRNA epigenetic alterations: predicting biomarkers and therapeutic targets in human diseases , 2008, Clinical genetics.

[77]  J. Komorowski,et al.  Kcnq1ot1 antisense noncoding RNA mediates lineage-specific transcriptional silencing through chromatin-level regulation. , 2008, Molecular cell.

[78]  R. Corn,et al.  Multiplexed detection methods for profiling microRNA expression in biological samples. , 2008, Angewandte Chemie.

[79]  Stefan Lorkowski,et al.  Susceptibility to coronary artery disease and diabetes is encoded by distinct, tightly linked SNPs in the ANRIL locus on chromosome 9p. , 2008, Human molecular genetics.

[80]  S. Sunkin,et al.  Specific expression of long noncoding RNAs in the mouse brain , 2008, Proceedings of the National Academy of Sciences.

[81]  S. Kauppinen,et al.  LNA-mediated microRNA silencing in non-human primates , 2008, Nature.

[82]  Torsten Haferlach,et al.  Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin , 2008, Proceedings of the National Academy of Sciences.

[83]  A. López-Guillermo,et al.  MicroRNA expression profiling in classic Hodgkin lymphoma. , 2008, Blood.

[84]  A. Hoffman,et al.  CTCF Regulates Allelic Expression of Igf2 by Orchestrating a Promoter-Polycomb Repressive Complex 2 Intrachromosomal Loop , 2008, Molecular and Cellular Biology.

[85]  J. Stenvang,et al.  MicroRNAs as targets for antisense-based therapeutics , 2008, Expert opinion on biological therapy.

[86]  A. Visel,et al.  Ultraconservation identifies a small subset of extremely constrained developmental enhancers , 2008, Nature Genetics.

[87]  T. Morgan,et al.  Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of β-secretase , 2008, Nature Medicine.

[88]  Karen L. Mohlke,et al.  INK4/ARF Transcript Expression Is Associated with Chromosome 9p21 Variants Linked to Atherosclerosis , 2009, PloS one.

[89]  G. Tonini,et al.  Transcribed-ultra conserved region expression is associated with outcome in high-risk neuroblastoma , 2009, BMC Cancer.

[90]  Gideon Rechavi,et al.  Diagnostic assay based on hsa-miR-205 expression distinguishes squamous from nonsquamous non-small-cell lung carcinoma. , 2009, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[91]  Alexander Deiters,et al.  Small Molecule Modifiers of the microRNA and RNA Interference Pathway , 2010, The AAPS Journal.

[92]  Ralf Küppers,et al.  The biology of Hodgkin's lymphoma , 2009, Nature Reviews Cancer.

[93]  D. Catalucci,et al.  MicroRNAs in Cardiovascular Biology and Heart Disease , 2009, Circulation. Cardiovascular genetics.

[94]  George A. Calin,et al.  MicroRNAs — the micro steering wheel of tumour metastases , 2009, Nature Reviews Cancer.

[95]  A. Schetter,et al.  MiR-21 is an EGFR-regulated anti-apoptotic factor in lung cancer in never-smokers , 2009, Proceedings of the National Academy of Sciences.

[96]  Yiping Zhao,et al.  Quantitative Surface-Enhanced Raman Spectroscopy Based Analysis of MicroRNA Mixtures , 2009, Applied spectroscopy.

[97]  Lei Han,et al.  MicroRNA-21 inhibitor sensitizes human glioblastoma cells U251 (PTEN-mutant) and LN229 (PTEN-wild type) to taxol , 2010, BMC Cancer.

[98]  S. Hébert,et al.  Alterations of the microRNA network cause neurodegenerative disease , 2009, Trends in Neurosciences.

[99]  Kaleb M. Pauley,et al.  MicroRNA in autoimmunity and autoimmune diseases. , 2009, Journal of autoimmunity.

[100]  X. Breakefield,et al.  Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer , 2009, British Journal of Cancer.

[101]  J. Rinn,et al.  Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression , 2009, Proceedings of the National Academy of Sciences.

[102]  Marilyn E Morris,et al.  MicroRNA-328 Negatively Regulates the Expression of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Cancer Cells , 2009, Molecular Pharmacology.

[103]  Cheng He,et al.  MicroRNA-21 targets LRRFIP1 and contributes to VM-26 resistance in glioblastoma multiforme , 2009, Brain Research.

[104]  Abraham Hochberg,et al.  Highly upregulated in liver cancer noncoding RNA is overexpressed in hepatic colorectal metastasis , 2009, European journal of gastroenterology & hepatology.

[105]  P. Radice,et al.  SNPs in ultraconserved elements and familial breast cancer risk. , 2009, Carcinogenesis.

[106]  C. Ambrosone,et al.  Novel genetic variants in microRNA genes and familial breast cancer , 2009, International journal of cancer.

[107]  P. Bugert,et al.  A genetic variant in the pre-miR-27a oncogene is associated with a reduced familial breast cancer risk , 2010, Breast Cancer Research and Treatment.

[108]  Sandra B. Munro,et al.  Detection of Cancer with Serum miRNAs on an Oligonucleotide Microarray , 2009, PloS one.

[109]  K. Ohuchida,et al.  MicroRNA-21 modulates biological functions of pancreatic cancer cells including their proliferation, invasion, and chemoresistance , 2009, Molecular Cancer Therapeutics.

[110]  J. Mattick,et al.  Long non-coding RNAs: insights into functions , 2009, Nature Reviews Genetics.

[111]  Ajay Agarwal,et al.  Label-free direct detection of MiRNAs with silicon nanowire biosensors. , 2009, Biosensors & bioelectronics.

[112]  Hongbing Shen,et al.  Functional variant in microRNA‐196a2 contributes to the susceptibility of congenital heart disease in a Chinese population , 2009, Human mutation.

[113]  S. Deo,et al.  Trends in microRNA detection , 2009, Analytical and bioanalytical chemistry.

[114]  W. Catalona,et al.  Common variants in 8q24 are associated with risk for prostate cancer and tumor aggressiveness in men of European ancestry , 2009, The Prostate.

[115]  G. Calin,et al.  MicroRNAs and Ultraconserved Genes as Diagnostic Markers and Therapeutic Targets in Cancer and Cardiovascular Diseases , 2010, Journal of cardiovascular translational research.

[116]  D. Brown,et al.  The promise of microRNA replacement therapy. , 2010, Cancer research.

[117]  B. Mittal,et al.  Common genetic variants in pre-microRNAs and risk of gallbladder cancer in North Indian population , 2010, Journal of Human Genetics.

[118]  David Galas,et al.  Surface plasmon resonance biosensor for rapid label-free detection of microribonucleic acid at subfemtomole level. , 2010, Analytical chemistry.

[119]  Vladimir Benes,et al.  Expression profiling of microRNA using real-time quantitative PCR, how to use it and what is available. , 2010, Methods.

[120]  Yuanjia Tang,et al.  MicroRNA-21 and MicroRNA-148a Contribute to DNA Hypomethylation in Lupus CD4+ T Cells by Directly and Indirectly Targeting DNA Methyltransferase 1 , 2010, The Journal of Immunology.

[121]  Yong-Tae Kim,et al.  Identification of MicroRNA-21 as a Biomarker for Chemoresistance and Clinical Outcome Following Adjuvant Therapy in Resectable Pancreatic Cancer , 2010, PloS one.

[122]  Bin Wang,et al.  Correlation between pre-miR-146a C/G polymorphism and gastric cancer risk in Chinese population. , 2010, World journal of gastroenterology.

[123]  D. Lewis,et al.  Flow cytometric analysis of circulating microparticles in plasma , 2010, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[124]  H. Heneghan,et al.  Circulating miRNA signatures: promising prognostic tools for cancer. , 2010, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[125]  R. Wong,et al.  Direct quantification of single-molecules of microRNA by total internal reflection fluorescence microscopy. , 2010, Analytical chemistry.

[126]  Y. Ruan,et al.  Genome-wide analysis reveals methyl-CpG–binding protein 2–dependent regulation of microRNAs in a mouse model of Rett syndrome , 2010, Proceedings of the National Academy of Sciences.

[127]  Ugo Boggi,et al.  MicroRNA-21 in pancreatic cancer: correlation with clinical outcome and pharmacologic aspects underlying its role in the modulation of gemcitabine activity. , 2010, Cancer research.

[128]  R. Aharonov,et al.  A diagnostic assay based on microRNA expression accurately identifies malignant pleural mesothelioma. , 2010, The Journal of molecular diagnostics : JMD.

[129]  Jingmin Jin,et al.  Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors. , 2010, Nature nanotechnology.

[130]  B. Blencowe,et al.  The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. , 2010, Molecular cell.

[131]  X. Yao,et al.  Involvement of microRNA-21 in mediating chemo-resistance to docetaxel in androgen-independent prostate cancer PC3 cells , 2010, Acta Pharmacologica Sinica.

[132]  P. Pandolfi,et al.  A coding-independent function of gene and pseudogene mRNAs regulates tumour biology , 2010, Nature.

[133]  R. Bernards.  It's Diagnostics, Stupid , 2010, Cell.

[134]  Massimo Negrini,et al.  Micromarkers: miRNAs in cancer diagnosis and prognosis , 2010, Expert review of molecular diagnostics.

[135]  J. Weidhaas,et al.  MicroRNA binding site polymorphisms as biomarkers of cancer risk , 2010, Expert review of molecular diagnostics.

[136]  Alain Laederach,et al.  Disease-Associated Mutations That Alter the RNA Structural Ensemble , 2010, PLoS genetics.

[137]  G. Schuler,et al.  ANRIL Expression Is Associated With Atherosclerosis Risk at Chromosome 9p21 , 2010, Arteriosclerosis, thrombosis, and vascular biology.

[138]  M. Esteller,et al.  CpG island hypermethylation-associated silencing of non-coding RNAs transcribed from ultraconserved regions in human cancer , 2010, Oncogene.

[139]  M. Fabbri.  miRNAs as molecular biomarkers of cancer , 2010, Expert review of molecular diagnostics.

[140]  H. Benjamin,et al.  Accurate Classification of Non–Small Cell Lung Carcinoma Using a Novel MicroRNA-Based Approach , 2010, Clinical Cancer Research.

[141]  Wei Zhang,et al.  A functional polymorphism in MSMB gene promoter is associated with prostate cancer risk and serum MSMB expression , 2010, The Prostate.

[142]  S. Volinia,et al.  Non-coding RNAs: a key to future personalized molecular therapy? , 2010, Genome Medicine.

[143]  C. Croce,et al.  Non-codingRNA sequence variations in human chronic lymphocytic leukemia and colorectal cancer. , 2010, Carcinogenesis.

[144]  C. Croce,et al.  Targeting microRNAs in cancer: rationale, strategies and challenges , 2010, Nature Reviews Drug Discovery.

[145]  Wei Zhang,et al.  A functional polymorphism in Pre‐miR‐146a gene is associated with prostate cancer risk and mature miR‐146a expression in vivo , 2010, The Prostate.

[146]  William R. Jeck,et al.  Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk , 2010, PLoS genetics.

[147]  Bernard Keavney,et al.  Chromosome 9p21 SNPs Associated with Multiple Disease Phenotypes Correlate with ANRIL Expression , 2010, PLoS genetics.

[148]  T. Derrien,et al.  Long Noncoding RNAs with Enhancer-like Function in Human Cells , 2010, Cell.

[149]  J Ma,et al.  MicroRNA and drug resistance , 2010, Cancer Gene Therapy.

[150]  Profiling of pre-micro RNAs and microRNAs using quantitative real-time PCR (qPCR) arrays. , 2010, Journal of visualized experiments : JoVE.

[151]  Jessica A. Weber,et al.  The microRNA spectrum in 12 body fluids. , 2010, Clinical chemistry.

[152]  Lisa E. Gralinski,et al.  Unique Signatures of Long Noncoding RNA Expression in Response to Virus Infection and Altered Innate Immune Signaling , 2010, mBio.

[153]  M. Caligiuri,et al.  miR-328 Functions as an RNA Decoy to Modulate hnRNP E2 Regulation of mRNA Translation in Leukemic Blasts , 2010, Cell.

[154]  J. Mattick,et al.  Long non-coding RNAs in nervous system function and disease , 2010, Brain Research.

[155]  J. Kohtz,et al.  Regulatory long non-coding RNAs and neuronal disorders , 2010, Physiology & Behavior.

[156]  Howard Y. Chang,et al.  Long noncoding RNA HOTAIR reprograms chromatin state to promote cancer metastasis , 2010, Nature.

[157]  Kerang Zhang,et al.  MicroRNAs and target site screening reveals a pre-microRNA-30e variant associated with schizophrenia , 2010, Schizophrenia Research.

[158]  M. Weiner,et al.  Cerebrospinal fluid and plasma biomarkers in Alzheimer disease , 2010, Nature Reviews Neurology.

[159]  Michael Q. Zhang,et al.  A long nuclear‐retained non‐coding RNA regulates synaptogenesis by modulating gene expression , 2010, EMBO Journal.

[160]  S. Dacic,et al.  miRNA expression profiling of lung adenocarcinomas: correlation with mutational status , 2010, Modern Pathology.

[161]  Kazuhiro Yoshida,et al.  Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysis. , 2010, The Lancet. Oncology.

[162]  Peter A. Jones,et al.  Epigenetics in cancer. , 2010, Carcinogenesis.

[163]  F. Marincola,et al.  Inherited polymorphisms in the RNA-mediated interference machinery affect microRNA expression and lung cancer survival , 2010, British Journal of Cancer.

[164]  Jiayi Wang,et al.  CREB up-regulates long non-coding RNA, HULC expression through interaction with microRNA-372 in liver cancer , 2010, Nucleic acids research.

[165]  N. Rosenfeld,et al.  Accurate molecular classification of renal tumors using microRNA expression. , 2010, The Journal of molecular diagnostics : JMD.

[166]  H. Heneghan,et al.  MiRNAs as biomarkers and therapeutic targets in cancer. , 2010, Current opinion in pharmacology.

[167]  S. Kauppinen,et al.  Therapeutic Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus Infection , 2010, Science.

[168]  J. Rinn,et al.  A Large Intergenic Noncoding RNA Induced by p53 Mediates Global Gene Repression in the p53 Response , 2010, Cell.

[169]  S. Abramson,et al.  The role of microRNA in rheumatoid arthritis and other autoimmune diseases. , 2010, Clinical immunology.

[170]  Jessica A. Weber,et al.  Export of microRNAs and microRNA-protective protein by mammalian cells , 2010, Nucleic acids research.

[171]  Bin Zhou,et al.  Common genetic polymorphisms in pre-microRNAs were associated with increased risk of dilated cardiomyopathy. , 2010, Clinica chimica acta; international journal of clinical chemistry.

[172]  Wei Xiong,et al.  MicroRNA-125b Confers the Resistance of Breast Cancer Cells to Paclitaxel through Suppression of Pro-apoptotic Bcl-2 Antagonist Killer 1 (Bak1) Expression* , 2010, The Journal of Biological Chemistry.

[173]  M. Korc,et al.  MicroRNA-10b Expression Correlates with Response to Neoadjuvant Therapy and Survival in Pancreatic Ductal Adenocarcinoma , 2011, Clinical Cancer Research.

[174]  M. Rodicio,et al.  Analytical aspects of microRNA in diagnostics: a review. , 2011, Analytica chimica acta.

[175]  Hua Zhang,et al.  Upregulation of microRNA-125b contributes to leukemogenesis and increases drug resistance in pediatric acute promyelocytic leukemia , 2011, Molecular Cancer.

[176]  Inyoul Lee,et al.  Extracellular microRNA: a new source of biomarkers. , 2011, Mutation research.

[177]  Yusuke Nakamura,et al.  Association of a novel long non‐coding RNA in 8q24 with prostate cancer susceptibility , 2011, Cancer science.

[178]  Howard Y. Chang,et al.  Long noncoding RNAs and human disease. , 2011, Trends in cell biology.

[179]  Peng Wu,et al.  An enzyme-linked assay for the rapid quantification of microRNAs based on the viral suppressor of RNA silencing protein p19. , 2011, Analytical biochemistry.

[180]  H. Hwu,et al.  MicroRNA Expression Aberration as Potential Peripheral Blood Biomarkers for Schizophrenia , 2011, PloS one.

[181]  Yunping Lu 卢运萍,et al.  miR-125b confers resistance of ovarian cancer cells to cisplatin by targeting pro-apoptotic Bcl-2 antagonist killer 1 , 2011, Journal of Huazhong University of Science and Technology [Medical Sciences].

[182]  Howard Y. Chang,et al.  A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression , 2011, Nature.

[183]  Keith W. Jones,et al.  Impact of Cellular miRNAs on Circulating miRNA Biomarker Signatures , 2011, PloS one.

[184]  Min-hui Yang,et al.  MALAT-1: a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis. , 2011, International journal of oncology.

[185]  Laura Beretta,et al.  Circulating MicroRNAs in Patients with Chronic Hepatitis C and Non-Alcoholic Fatty Liver Disease , 2011, PloS one.

[186]  J. Olson,et al.  A functional polymorphism in the pre-miR-146a gene is associated with risk and prognosis in adult glioma , 2011, Journal of Neuro-Oncology.

[187]  Catherine L Jopling,et al.  The role of microRNAs in viral infection. , 2011, Progress in molecular biology and translational science.

[188]  Carolyn J. Brown,et al.  The functional role of long non-coding RNA in human carcinomas , 2011, Molecular Cancer.

[189]  Jørgen Kjems,et al.  miRNA‐dependent gene silencing involving Ago2‐mediated cleavage of a circular antisense RNA , 2011, The EMBO journal.

[190]  Yurong Fu,et al.  Circulating MicroRNAs in Patients with Active Pulmonary Tuberculosis , 2011, Journal of Clinical Microbiology.

[191]  E. Bandrés,et al.  Role of primary miRNA polymorphic variants in metastatic colon cancer patients treated with 5-fluorouracil and irinotecan , 2011, The Pharmacogenomics Journal.

[192]  K. Vickers,et al.  MicroRNAs are Transported in Plasma and Delivered to Recipient Cells by High-Density Lipoproteins , 2011, Nature Cell Biology.

[193]  R. Aharonov,et al.  A second-generation microRNA-based assay for diagnosing tumor tissue origin. , 2011, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[194]  E. Kroh,et al.  Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma , 2011, Proceedings of the National Academy of Sciences.

[195]  Kris Richardson,et al.  A genome-wide survey for SNPs altering microRNA seed sites identifies functional candidates in GWAS , 2011, BMC Genomics.

[196]  B. Norrving,et al.  Global atlas on cardiovascular disease prevention and control. , 2011 .

[197]  Giuliano Binetti,et al.  Cerebrospinal Fluid Biomarkers for Alzheimer’s Disease: The Present and the Future , 2011, Neurodegenerative Diseases.

[198]  Xiaofeng Ding,et al.  A miR-200b/200c/429-Binding Site Polymorphism in the 3′ Untranslated Region of the AP-2α Gene Is Associated with Cisplatin Resistance , 2011, PloS one.

[199]  Zhiqiang Gao,et al.  Amplified detection of microRNA based on ruthenium oxide nanoparticle-initiated deposition of an insulating film. , 2011, Analytical chemistry.

[200]  G. Hirokawa,et al.  Plasma miR-124 as a biomarker for cerebral infarction. , 2011, Biomedical research.

[201]  Sergio Verjovski-Almeida,et al.  Long noncoding intronic RNAs are differentially expressed in primary and metastatic pancreatic cancer , 2011, Molecular Cancer.

[202]  G. Calin,et al.  MicroRNAs in body fluids—the mix of hormones and biomarkers , 2011, Nature Reviews Clinical Oncology.

[203]  John T. Wei,et al.  Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression , 2011, Nature Biotechnology.

[204]  E. Kroh,et al.  Blood Cell Origin of Circulating MicroRNAs: A Cautionary Note for Cancer Biomarker Studies , 2011, Cancer Prevention Research.

[205]  New microRNA-based diagnostic test for lung cancer classification. , 2011, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[206]  Andrew E. Bruno,et al.  miRdSNP: a database of disease-associated SNPs and microRNA target sites on 3'UTRs of human genes , 2012, BMC Genomics.

[207]  P. Tien,et al.  Circulating microRNAs in hepatitis B virus–infected patients , 2011, Journal of viral hepatitis.

[208]  Hongbing Shen,et al.  Genetic polymorphisms in the precursor MicroRNA flanking region and non-small cell lung cancer survival. , 2011, American journal of respiratory and critical care medicine.

[209]  Ferdinando Di Cunto,et al.  Coding-Independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous mRNAs , 2011, Cell.

[210]  F. Zhang,et al.  Long non-coding RNA MALAT-1 overexpression predicts tumor recurrence of hepatocellular carcinoma after liver transplantation , 2012, Medical Oncology.

[211]  M. Satoh,et al.  A cellular microRNA, let-7i, is a novel biomarker for clinical outcome in patients with dilated cardiomyopathy. , 2011, Journal of cardiac failure.

[212]  Xi Yang,et al.  Using microRNA as Biomarkers of Drug-Induced Liver Injury , 2011 .

[213]  Wei Zhang,et al.  microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells. , 2011, Oncology reports.

[214]  Guo-Yuan Yang,et al.  MicroRNA-210 as a novel blood biomarker in acute cerebral ischemia. , 2011, Frontiers in bioscience.

[215]  Y. Liu,et al.  MicroRNA-134 as a potential plasma biomarker for the diagnosis of acute pulmonary embolism , 2011, Journal of Translational Medicine.

[216]  Emily A. Vucic,et al.  Human Cancer Long Non-Coding RNA Transcriptomes , 2011, PloS one.

[217]  Q. Li,et al.  Large Intervening Non-Coding RNA HOTAIR is Associated with Hepatocellular Carcinoma Progression , 2011, The Journal of international medical research.

[218]  S. Koschmieder,et al.  The Long Noncoding MALAT-1 RNA Indicates a Poor Prognosis in Non-small Cell Lung Cancer and Induces Migration and Tumor Growth , 2011, Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer.

[219]  T. Shioi,et al.  MicroRNA, emerging role as a biomarker of heart failure. , 2011, Circulation journal : official journal of the Japanese Circulation Society.

[220]  D. Cacchiarelli,et al.  A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA , 2011, Cell.

[221]  J. Bauersachs,et al.  Diagnostic and prognostic impact of six circulating microRNAs in acute coronary syndrome. , 2011, Journal of molecular and cellular cardiology.

[222]  D. Omahen.  MicroRNA and Diseases of the Nervous System , 2011, Neurosurgery.

[223]  Xuerui Yang,et al.  An Extensive MicroRNA-Mediated Network of RNA-RNA Interactions Regulates Established Oncogenic Pathways in Glioblastoma , 2011, Cell.

[224]  P. A. van den Brandt,et al.  A Let-7 MicroRNA SNP in the KRAS 3′UTR Is Prognostic in Early-Stage Colorectal Cancer , 2011, Clinical Cancer Research.

[225]  A. Chinnaiyan,et al.  The emergence of lncRNAs in cancer biology. , 2011, Cancer discovery.

[226]  Alicia Algeciras-Schimnich,et al.  Analysis of circulating microRNA: preanalytical and analytical challenges. , 2011, Clinical chemistry.

[227]  S. Rai,et al.  MicroRNA signature of cis-platin resistant vs. cis-platin sensitive ovarian cancer cell lines , 2011, Journal of ovarian research.

[228]  Hushan Yang,et al.  Genetic Polymorphisms in Pre-microRNA Genes as Prognostic Markers of Colorectal Cancer , 2011, Cancer Epidemiology, Biomarkers & Prevention.

[229]  S. Miyano,et al.  Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. , 2011, Cancer research.

[230]  Q. Lu,et al.  MicroRNA-126 regulates DNA methylation in CD4+ T cells and contributes to systemic lupus erythematosus by targeting DNA methyltransferase 1. , 2011, Arthritis and rheumatism.

[231]  M. Leonard,et al.  Circulating MicroRNA Is a Biomarker of Pediatric Crohn Disease , 2011, Journal of pediatric gastroenterology and nutrition.

[232]  E. Olson,et al.  Pervasive roles of microRNAs in cardiovascular biology , 2011, Nature.

[233]  M. Esteller,et al.  A precursor microRNA in a cancer cell nucleus , 2011, Cell cycle.

[234]  S. Ahmed,et al.  MicroRNA, a new paradigm for understanding immunoregulation, inflammation, and autoimmune diseases. , 2011, Translational research : the journal of laboratory and clinical medicine.

[235]  X. Breakefield,et al.  Brain Tumor Microvesicles: Insights into Intercellular Communication in the Nervous System , 2011, Cellular and Molecular Neurobiology.

[236]  P. Pandolfi,et al.  In Vivo Identification of Tumor- Suppressive PTEN ceRNAs in an Oncogenic BRAF-Induced Mouse Model of Melanoma , 2011, Cell.

[237]  Yong Wang,et al.  Nanopore-based detection of circulating microRNAs in lung cancer patients , 2011, Nature nanotechnology.

[238]  Y. Mo,et al.  Detection of microRNAs in cultured cells and paraffin-embedded tissue specimens by in situ hybridization. , 2011, Methods in molecular biology.

[239]  B. Burwinkel,et al.  Characterization of extracellular circulating microRNA , 2011, Nucleic acids research.

[240]  M. Sabbagh,et al.  BACE1 as a potential biomarker for Alzheimer's disease. , 2011, Journal of Alzheimer's disease : JAD.

[241]  C. Glass,et al.  Reprogramming Transcription via Distinct Classes of Enhancers Functionally Defined by eRNA , 2011, Nature.

[242]  D. Girelli,et al.  A polymorphism in the chromosome 9p21 ANRIL locus is associated to Philadelphia positive acute lymphoblastic leukemia. , 2011, Leukemia research.

[243]  M. Esteller.  Non-coding RNAs in human disease , 2011, Nature Reviews Genetics.

[244]  M. Kitagawa,et al.  Long non-coding RNA ANRIL is required for the PRC2 recruitment to and silencing of p15INK4B tumor suppressor gene , 2011, Oncogene.

[245]  Howard Y. Chang,et al.  Molecular mechanisms of long noncoding RNAs. , 2011, Molecular cell.

[246]  T. Ørntoft,et al.  Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs , 2011, BMC Genomics.

[247]  D. Goldstein.  Growth of genome screening needs debate , 2011, Nature.

[248]  E. Li,et al.  Serum and urinary free microRNA level in patients with systemic lupus erythematosus , 2011, Lupus.

[249]  Fabio Martelli,et al.  microRNAs as peripheral blood biomarkers of cardiovascular disease. , 2011, Vascular pharmacology.

[250]  P. Pandolfi,et al.  A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language? , 2011, Cell.

[251]  Holger Sültmann,et al.  Circulating miRNAs are correlated with tumor progression in prostate cancer , 2011, International journal of cancer.

[252]  Nan Shen,et al.  A Functional Variant in MicroRNA-146a Promoter Modulates Its Expression and Confers Disease Risk for Systemic Lupus Erythematosus , 2011, PLoS genetics.

[253]  Haiyang Xie,et al.  Overexpression of Long Non-coding RNA HOTAIR Predicts Tumor Recurrence in Hepatocellular Carcinoma Patients Following Liver Transplantation , 2011, Annals of Surgical Oncology.

[254]  N. Jablonski,et al.  Circulating microRNAs involved in multiple sclerosis , 2012, Molecular Biology Reports.

[255]  Guihua Sun,et al.  MicroRNAs and their potential involvement in HIV infection. , 2011, Trends in pharmacological sciences.

[256]  R. Aharonov,et al.  A Second-Generation MicroRNA-Based Assay for Diagnosing Tumor Tissue Origin , 2012 .

[257]  A. Wakamatsu,et al.  Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals , 2012, Genome research.

[258]  T. Luedde,et al.  Micro-RNA Profiling in Human Serum Reveals Compartment-Specific Roles of miR-571 and miR-652 in Liver Cirrhosis , 2012, PloS one.

[259]  Wan L. Lam,et al.  Mechanistic Roles of Noncoding RNAs in Lung Cancer Biology and Their Clinical Implications , 2012, Genetics research international.

[260]  Hai-yan Lin,et al.  Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies. , 2012, Reproduction.

[261]  S. Vasudevan.  Posttranscriptional Upregulation by MicroRNAs , 2012, Wiley interdisciplinary reviews. RNA.

[262]  Chad A Mirkin,et al.  Scanometric microRNA array profiling of prostate cancer markers using spherical nucleic acid-gold nanoparticle conjugates. , 2012, Analytical chemistry.

[263]  D. Bishop,et al.  The role of microRNA-binding site polymorphisms in DNA repair genes as risk factors for bladder cancer and breast cancer and their impact on radiotherapy outcomes , 2011, Carcinogenesis.

[264]  R. Bast,et al.  Plasma microRNA 210 levels correlate with sensitivity to trastuzumab and tumor presence in breast cancer patients , 2012, Cancer.

[265]  Y. You,et al.  MicroRNA-125b-2 confers human glioblastoma stem cells resistance to temozolomide through the mitochondrial pathway of apoptosis. , 2011, International journal of oncology.

[266]  J. Mendell,et al.  MicroRNAs in Stress Signaling and Human Disease , 2012, Cell.

[267]  Evelyn Wenkel,et al.  Circulating Micro-RNAs as Potential Blood-Based Markers for Early Stage Breast Cancer Detection , 2012, PloS one.

[268]  S. Verjovski-Almeida,et al.  Perspectives of Long Non-Coding RNAs in Cancer Diagnostics , 2012, Front. Gene..

[269]  S. Zeuzem,et al.  58 FINAL RESULTS – RANDOMIZED, DOUBLE-BLIND, PLACEBO-CONTROLLED SAFETY, ANTI-VIRAL PROOF-OF-CONCEPT STUDY OF MIRAVIRSEN, AN OLIGONUCLEOTIDE TARGETING MIR-122, IN TREATMENT-NAIVE PATIENTS WITH GENOTYPE 1 CHRONIC HCV INFECTION , 2012 .

[270]  Joon Cheol Park,et al.  Plasma components affect accuracy of circulating cancer-related microRNA quantitation. , 2012, The Journal of molecular diagnostics : JMD.

[271]  Q. Cai,et al.  A MicroRNA-7 Binding Site Polymorphism in HOXB5 Leads to Differential Gene Expression in Bladder Cancer , 2012, PloS one.

[272]  D. Mozaffarian,et al.  Executive summary: heart disease and stroke statistics--2012 update: a report from the American Heart Association. , 2012, Circulation.

[273]  T. Thum.  MicroRNA therapeutics in cardiovascular medicine , 2012, EMBO molecular medicine.

[274]  F. Hu,et al.  Variants on Chromosome 9p21.3 Correlated With ANRIL Expression Contribute to Stroke Risk and Recurrence in a Large Prospective Stroke Population , 2012, Stroke.

[275]  C. Mathieu,et al.  Decreased miR-181a expression in monocytes of obese patients is associated with the occurrence of metabolic syndrome and coronary artery disease. , 2012, The Journal of clinical endocrinology and metabolism.

[276]  Botao Zhao,et al.  A simple and fast method for profiling microRNA expression from low‐input total RNA by microarray , 2012, IUBMB life.

[277]  Anirban P. Mitra,et al.  A Central Role for Long Non-Coding RNA in Cancer , 2011, Front. Gene..

[278]  Tanja Kunej,et al.  Catalog of MicroRNA Seed Polymorphisms in Vertebrates , 2012, PloS one.

[279]  Hongjiang Wang,et al.  Circulating MiR-125b as a Marker Predicting Chemoresistance in Breast Cancer , 2012, PloS one.

[280]  R. Gambari,et al.  Involvement of miRNA in erythroid differentiation. , 2012, Epigenomics.

[281]  Xi Chen,et al.  Serum microRNA profiling and breast cancer risk: the use of miR-484/191 as endogenous controls. , 2012, Carcinogenesis.

[282]  T. Mayer,et al.  Nanoresonator chip-based RNA sensor strategy for detection of circulating tumor cells: response using PCA3 as a prostate cancer marker. , 2012, Nanomedicine : nanotechnology, biology, and medicine.

[283]  M. Toyota,et al.  Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors. , 2012, Cancer research.

[284]  F. Slack,et al.  Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization. , 2012, BioTechniques.

[285]  G. Jicha,et al.  Blood serum miRNA: Non-invasive biomarkers for Alzheimer's disease , 2012, Experimental Neurology.

[286]  M. Gazouli,et al.  Circulating MicroRNA in inflammatory bowel disease. , 2012, Journal of Crohn's & colitis.

[287]  A. Tijsen,et al.  Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease? , 2012, Circulation research.

[288]  Rory Johnson.  Long non-coding RNAs in Huntington's disease neurodegeneration , 2012, Neurobiology of Disease.

[289]  Gabriel Wong,et al.  Stem Cell Marker (Nanog) and Stat-3 Signaling Promote MicroRNA-21 Expression and Chemoresistance in Hyaluronan/CD44-activated Head and Neck Squamous Cell Carcinoma Cells , 2011, Oncogene.

[290]  J. Weidhaas,et al.  MicroRNA Binding-Site Polymorphisms as Potential Biomarkers of Cancer Risk , 2010, Molecular Diagnosis & Therapy.

[291]  MicroRNA-21 Inhibitor Sensitizes Human Glioblastoma U251 Stem Cells to Chemotherapeutic Drug Temozolomide , 2012, Journal of Molecular Neuroscience.

[292]  C. Barbato,et al.  Targeting microRNAs in neurons: Tools and perspectives , 2012, Experimental Neurology.

[293]  K. Mimori,et al.  MicroRNA-10b is a Prognostic Indicator in Colorectal Cancer and Confers Resistance to the Chemotherapeutic Agent 5-Fluorouracil in Colorectal Cancer Cells , 2012, Annals of Surgical Oncology.

[294]  L. Defrancesco.  Life Technologies promises $1,000 genome , 2012, Nature Biotechnology.

[295]  L. Holdt,et al.  Recent studies of the human chromosome 9p21 locus, which is associated with atherosclerosis in human populations. , 2012, Arteriosclerosis, thrombosis, and vascular biology.

[296]  P. Jin,et al.  Role of noncoding RNAs in trinucleotide repeat neurodegenerative disorders , 2012, Experimental Neurology.

[297]  Kelly L. Robertson,et al.  Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection , 2012, Journal of visualized experiments : JoVE.

[298]  J. Mattick,et al.  Genome-wide analysis of long noncoding RNA stability , 2012, Genome research.

[299]  Jing Zhang,et al.  Highly sensitive multiple microRNA detection based on fluorescence quenching of graphene oxide and isothermal strand-displacement polymerase reaction. , 2012, Analytical chemistry.

[300]  Ofer Isakov,et al.  Novel insight into the non-coding repertoire through deep sequencing analysis , 2012, Nucleic acids research.

[301]  Demin Duan,et al.  Direct microRNA detection with universal tagged probe and time-resolved fluorescence technology. , 2012, Biosensors & bioelectronics.

[302]  R. Spizzo,et al.  Long non-coding RNAs and cancer: a new frontier of translational research? , 2012, Oncogene.

[303]  T. Thum,et al.  MicroRNA-based therapeutic approaches in the cardiovascular system. , 2012, Cardiovascular therapeutics.

[304]  T. Thum,et al.  Circulating and urinary microRNAs in kidney disease. , 2012, Clinical journal of the American Society of Nephrology : CJASN.

[305]  Juan Wen,et al.  A Genetic Variant in Long Non-Coding RNA HULC Contributes to Risk of HBV-Related Hepatocellular Carcinoma in a Chinese Population , 2012, PloS one.

[306]  A. Khalil,et al.  Emerging Roles for Long Non-Coding RNAs in Cancer and Neurological Disorders , 2011, Front. Gene..